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Organisme vivant modifié
(LMO)
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Insect-Resistant and Herbicide-Tolerant Maize
EN
4114
Oui
DP-ØØ4114-3
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Organisation :Pioneer Hi-Bred International Inc. ()Secteur privé (affaires et industrie)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, États-Unis d'AmériqueTéléphone : +1 515 535-3200,Télécopieur :E-mail :Site Web : www.pioneer.com/,
Maize (Zea mays) was modified for resistance to lepidoptera, including European corn borer (Ostrinia nubilalis), and coleoptera insect pests including corn rootworm pests, such as the western corn rootworm (Diabrotica virgifera virgifera), and tolerance to glufosinate-ammonium herbicide. To achieve resistance to lepidopteran insects, the maize expresses the cryIF gene from Bacillus thuringiensis. To achieve resistance to coleopteran insects, the maize expresses the cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis. The cry genes have a pore forming mode-of-action in the epithelial lining of feeding larvae; they produce a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve glufosinate tolerance, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-246-6 Organisme Zea mays (Maize, Corn, MAIZE)Cultures
Maize inbred line: PHWWE
EN
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DAS-59122-7 × DAS-Ø15Ø7-1 × MON-ØØ6Ø3-6 - Herculex XTRA™ Roundup Ready™ 2 maize| Pioneer Hi-Bred International Inc. | Résistance aux maladies et aux parasites (Insectes, Coléoptères, Chenille tisseuse (papillons et mites)), Résistance aux herbicides (Glufosinate, Glyphosate)
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DP-Ø32316-8 - Insect Resistant, Herbicide Tolerant Maize| Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites)), Résistance aux herbicides (Glufosinate)
PHP27118
EN
- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoteur
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-14994-9 Cry34Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Coléoptères)
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BCH-GENE-SCBD-100367-4 Proteinase inhibitor II gene terminator | Solanum tuberosum (Potato, SOLTU)Terminateur
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BCH-GENE-SCBD-100368-6 Peroxidase gene promoter | Triticum aestivum (Wheat)Promoteur
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BCH-GENE-SCBD-14995-8 Cry35Ab1 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Coléoptères)
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminateur
Information on the inserted DNA sequences
The transforming plasmid for maize DP-ØØ4114-3 contained a transfer DNA region comprising four gene cassettes: three expressing insecticidal proteins, cryIF, cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis, and one cassette used for positive transformant selection expressing the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT).
(1) The cryIF gene is regulated by the ubiquitin gene promoter from Zea mays and the ORF25 polyA terminator sequence from Agrobacterium tumefaciens. An intron from Zea mays (intron 1) was included to enhance expression of the cryIF sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(2) The cry34Ab1 gene is regulated by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. The intron 1 from Z. mays was included to enhance expression of the cry34Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(3) The cry35Ab1 gene is regulated peroxidase gene promoter from Triticum aestivum by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. An intron from Z. mays was included to enhance expression of the cry35Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter.
(4) The pat is used as a selectable marker for transformants, and the gene is regulated by the Cauliflower mosaic virus 35S promoter and terminator.
The transforming plasmid for maize DP-ØØ4114-3 contained a transfer DNA region comprising four gene cassettes: three expressing insecticidal proteins, cryIF, cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis, and one cassette used for positive transformant selection expressing the herbicide tolerance protein phosphinothricin N-acetyltransferase (PAT).
(1) The cryIF gene is regulated by the ubiquitin gene promoter from Zea mays and the ORF25 polyA terminator sequence from Agrobacterium tumefaciens. An intron from Zea mays (intron 1) was included to enhance expression of the cryIF sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(2) The cry34Ab1 gene is regulated by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. The intron 1 from Z. mays was included to enhance expression of the cry34Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(3) The cry35Ab1 gene is regulated peroxidase gene promoter from Triticum aestivum by the ubiquitin gene promoter from Z. mays and the proteinase inhibitor II terminator from Solanum tuberosum. An intron from Z. mays was included to enhance expression of the cry35Ab1 sequence from B. thuringiensis. High levels of transcription are expected due to the strong constitutive promoter.
(4) The pat is used as a selectable marker for transformants, and the gene is regulated by the Cauliflower mosaic virus 35S promoter and terminator.
Note
- The polyubiquitin gene promoter element is made up of the promoter region (900bp) and the 5’ untranslated region of the polyubiquitin gene (83 bp).
- Southern blot analyses indicated that a single, intact transformation cassette was inserted into the genome of 4114 maize with no integration of the backbone of plasmid PHP27118.
FR
- Nourriture pour les animaux
- Nourriture
- DP-ØØ4114-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
FR
FR
- EUginius: 4114 [URL] [ English ]
- ISAAA GM Approval database: 4114 [URL] [ English ]
- Patent (2011). Maize event DP-004114-3 and methods for detection thereof [PDF] ( US20110154523A1 ) [ English ]
- OECD BioTrack database: DP-ØØ4114-3 [URL] [ English ]
- FAO GM Foods Platform: DP-ØØ4114-3 [URL] [ English ]
- FSANZ Safety Assessment (A1106). Food derived from Herbicide-tolerant & Insect-protected Corn Line 4114 [PDF] ( Food Standards Australia New Zealand ) [ English ]
- EFSA (2018). Assessment of maize 4114 [PDF] ( food and feed uses, Regulation (EC) No 1829/2003 (application EFSA-GMO-NL-2014-123) ) [ English ]
- USDA APHIS (2013). Pioneer Petition for the Determination of Nonregulated Status for Insect-Resistant and Herbicide-Tolerant 4114 Maize [PDF] ( 11_244_01p ) [ English ]
- USDA-APHIS: Final Environmental Assessment (DP-004114-3) [PDF] ( 11_244_01p ) [ English ]
- USDA-APHIS: Plant Pest Risk Assessment for Pioneer 4114 Maize [PDF] ( 11_244_01p ) [ English ]
- Food and Chemical Toxicology (2013). Thirteen week rodent feeding study with grain from molecular stacked trait lepidopteran and coleopteran protected (DP-ØØ4114-3) maize [PDF] [ English ]
- CFIA Decision (DD 2013-98). Determination of the safety of Pioneer Hi-Bred Production Ltd.'s corn (Zea mays L.) event 4114 [URL] [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 10 | |
| Organisme vivant modifié | Related LMO(s) | 1 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 12 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 12 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 4 | |
