PV-GMIR13196 contains two separate T-DNAs that are each delineated by left and right border regions. The first T-DNA, designated as T-DNA I, contains the crylA.105 and cry2Ab2 expression cassettes, with genetic elements as indicated above.

The second T-DNA, designated as T-DNA II, contains marker genes that allow for simplified selection of transformed tissue. During transformation, both T-DNAs were inserted into the soybean genome. Subsequently, traditional breeding, segregation, selection and screening were used to isolate those plants that contain the crylA.105 and cry2Ab2 expression cassettes and do not contain the marker gene expression cassettes.

Characterization of the DNA insert in MON 87751 was conducted using a combination of sequencing, PCR, and bioinformatics. The results of this characterization demonstrate that MON 87751 contains one copy of T-DNA I containing the cry1A.105 and cry2Ab2 expression cassettes that is stably integrated at a single locus and is inherited according to Mendelian principles over multiple generations. No vector backbone sequences were detetcted