Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordCe document a été mis à jour. Il ne s’agit pas de la dernière version publiée. Cliquez ici pour voir la dernière version du fichier.
Organisme vivant modifié
(LMO)
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Roundup Ready™ maize
EN
NK603
Oui
MON-ØØ6Ø3-6
-
Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com,
The maize (Zea mays) was modified for tolerance to glyphosate herbicides. To achieve glyphosate tolerance, the maize expresses enzyme 5-enolpyruvylshikimate-3-phosphate synthase, encoded by the cp4 epsps gene from Agrobacterium tumefaciens strain CP4, which has reduced binding affinity for glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway in the presence of the herbicide.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
-
BCH-ORGA-SCBD-246-6 Organisme Zea mays (Maize, Corn, MAIZE)Cultures
FR
PV-ZMGT32
EN
- Pistolet biolistique / à particules
|
0.800 kb
|
|
0.600 kb
|
|
0.200 kb
|
|
1.400 kb
|
|
0.300 kb
|
|
0.600 kb
|
|
0.800 kb
|
|
0.200 kb
|
|
1.400 kb
|
|
0.300 kb
|
Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
-
BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
-
BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoteur
-
BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
-
BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
-
BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
-
BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
Information about the inserted DNA sequences
The DNA insert contains two adjacent genetic cassettes expressing the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium tumefaciens strain CP4. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cp4 epsps gene is under the regulation of the rice actin promoter (P-Ract1) and the rice actin intron (I-Ract1) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from Oryza sativa (I-Ract1) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
(2) The cp4 epsps gene is regulated by the enhanced Cauliflower mosaic virus 35S promoter (CaMV P-e35S) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from the Zea mays heat shock protein 70 (HSP70) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
Note
The DNA insert contains two adjacent genetic cassettes expressing the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium tumefaciens strain CP4. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cp4 epsps gene is under the regulation of the rice actin promoter (P-Ract1) and the rice actin intron (I-Ract1) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from Oryza sativa (I-Ract1) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
(2) The cp4 epsps gene is regulated by the enhanced Cauliflower mosaic virus 35S promoter (CaMV P-e35S) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from the Zea mays heat shock protein 70 (HSP70) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
Note
- The plasmid carrying the DNA transfer was digested by restriction enzyme MluI and only the fragments containing the two expression cassettes were inserted into the LM maize. The nptII gene encoding neomycin phosphotransferase II for kanamycin resistance and an origin of replication were not inserted.
- Maize line NK603 contains one insertion site containing a single copy of the linear DNA of PV-ZMGT32 used for transformation. Both cp4 epsps gene cassettes within the single insert which are intact.
- Promoter e35S is a 0.61Kb long sequence containing the promoter and leader for the CaMV 35S RNA containing the duplicated enhancer region.
FR
- Biocombustible
- Nourriture pour les animaux
- Nourriture
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database [ English ]
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
Glyphosate specifically binds to and inactivates the enzyme EPSPS, which is part of an important plant biochemical pathway called the shikimate pathway. The shikimate pathway is involved in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine and tryptophan, as well as other aromatic compounds. When conventional plants are treated with glyphosate they cannot produce the aromatic amino acids essential to their survival. The modified maize line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize. The EPSPS enzyme is present in all plants, bacteria and fungi, but not in animals, which do not synthesize their own aromatic amino acids. Thus, EPSPS is normally present in food derived from plant and microbial sources.
- EUginius: NK603 [URL] [ English ]
- OECD Biotrack database: NK603 [URL] [ English ]
- BATS (2003). Genetically Modified (GM) Crops: Molecular and Regulatory details, version 2 [PDF] [ English ]
- ANZFA (2002). A416 Final Assessment Report - Glyophaste-tolerant Corn Line NK603 [PDF] ( FSANZ, Food Standards Australia New Zealand, Australia New Zealand Food Authority ) [ English ]
- EFSA (2025). Assessment of maize NK603 [PDF] ( renewal authorisation, Regulation (EC) No 1829/2003 (dossier GMFF-2023-21250) ) [ English ]
- EU Commission FFID. Field trials for identification, characterization and agronomical evaluation for registration purposes in the list of O.E.V.V. vegetal corn varieties derived form NK603 line, tolerant to to glyphosate herbicide [URL] [ English ]
- Patent (2007). Corn event PV-ZMGT32(nk603) and compositions and methods for detection thereof [PDF] ( US20070292854A1 ) [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 59 | |
| Organisme vivant modifié | Related LMO(s) | 2 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 101 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 113 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 34 | |
