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Organisme vivant modifié (LMO)
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BCH-LMO-SCBD-14797-16   |   PDF   |   Imprimer   |  
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Décisions sur l’OVM Évaluations des risques  
published: 05 juin 2006 last updated: 13 juil. 2026
Identité de l’organisme vivant modifié
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Agrisure™ CB/LL
EN
Bt11 (X4334CBR, X4734CBR)
Oui
SYN-BTØ11-1
Maize (Zea mays) was modified for resistance to the Lepidoptera insect European corn borer (Ostrinia nubilalis) and tolerance to glufosinate-ammonium herbicides. To achieve resistance to the European corn borer, the maize expresses cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which have a pore forming mode-of-action in the epithelial lining of feeding larvae; cry1Ab produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve resistance to glufosinate, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase encoding gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
EN
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
FR
  • BCH-LMO-SCBD-45400-3
    Bt-10 Maize
    | Résistance aux antibiotiques (Ampicilline), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Pyrale du maïs (Ostrinia nubilalis)), Résistance aux herbicides (Glufosinate)
    Show detection method(s)
Caractéristiques du processus de modification
pZO1502 Derived from pUC18
EN
  • Transfert direct d’ADN
 
0.510 kb
 
 
0.470 kb
 
 
1.850 kb
 
 
0.250 kb
 
 
0.420 kb
 
 
0.180 kb
 
 
0.550 kb
 
 
0.250 kb
 
Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
  • BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)
    Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
  • BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminateur
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoteur
  • BCH-GENE-SCBD-103625-3 Alcohol dehydrogenase 1, intron 6 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-103867-2 Alcohol dehydrogenase 1, intron 2 | Zea mays (Maize, Corn, MAIZE)
    Intron
Information on the inserted DNA sequences
The DNA insert contained two genetic cassettes expressing the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes. Both cassettes were introduced by particle acceleration (biolistic) transformation.

(1) The cry1Ab gene is under the control of the 35S Cauliflower mosaic virus promoter and the Agrobacterium tumefaciens nopaline synthase gene terminator. An alcohol dehydrogenase 1 intron (intron 6) from Zea mays was included to enhance expression of the cry1Ab sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.

(2) The pat gene is regulated by the 35S promoter from Cauliflower mosaic virus and the nopaline synthase gene terminator from Agrobacterium tumefaciens. The Zea mays alcohol dehydrogenase 1 intron (intron 2) from was also included to enhance expression of the phosphinothricin N-acetyltransferase sequence from Streptomyces viridochromogenes. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.

Note
  • The plasmid carrying the DNA transfer was digested by restriction enzyme NotI and only the fragments containing the two expression cassettes were inserted into the LM maize. The AMPr gene encoding ampicillin resistance and an origin of replication were not inserted.
  • Southern blot analysis confirmed the absence of unwanted DNA fragments in the transformant generations.
  • The CaMV promoter associated with cry1Ab, was isolated from the CM1841 strain of CaMV using DdeI restriction digestion. However, the DdeI sites were converted into SacI sites.
  • The CaMV promoter associated with pat was isolated from the Cabb-S strain of CaMV (AluI to DdeI fragment) and subsequently modified.
  • The cry1Ab coding sequence encodes a truncated version corresponding to the N-terminal 615 amino acids of the full length Cry1Ab protein.
  • The cloning of pat did not result in any amino acid sequence changes in the parental line.
  • The nos terminator corresponds to the 423 to 678 basepairs of the nopaline synthase gene in A. tumefaciens.
EN
Caractéristiques de l’OVM
FR
  • Nourriture pour les animaux
  • Nourriture
Informations supplémentaires
Information on the inserted DNA sequences
  • 35S promoters derived from cauliflower mosaic virus (CaMV) and 35S-1 originated from the CM1841 isolate of CaMV as a 500 Ddel to Ddel fragment, subsequently converted to Sacl sites and 35S-2 originated from the Cabb-S strain of CaMV as a n Alul to Ddel fragments (ca 425bp), whose ends were subsequently modified.
  • Introns derived from the maize alcohol dehydrogenase 1S gene and were used to enhance heterologous gene expression.
  • Btk gene which is an altered version of the full length cry1A(b) gene of Bacillus thuringiensis var kurstaki HD-1. The Btk was obtained as a 1.8kb Nco-Bg/ll fragment. The truncated Btk protein is identical to the N-terminal 615 amino acids of the native Btk protein of 1155 amino acids
  • pat gene (phosphinotricin acetyl transferase) cloned from the soil microorganism, Streptomyces viridochromogenes strain Tu494.  Alteration did not result in any amino acid sequence changes.
  • nos terminator consisting of 423-678 of the nopaline synthase gene of Agrobacterium tumefaciens plus added restriction sites.

    Vector information
    Plasmid pZO1502 is the vector used for the transformation of Bt maize.  This is a derivative of plasmid pUC18. The plasmid has a molecular weight of 2.7 kb and contains the following sequences:
    - the prokaryotic gene bla (also called ampR) under a procaryotic promoter encoding β-lactamase, which confers resistance to ampicillin; it is used as a bacterial selectable marker;  
    - the gene lac Z, encoding a portion of a β-galactosidase, this gene is not functional; 
    - the pUC origin of replication derived from the plasmid pBR 322 carrying a mutation.
EN
Les fichiers qui font référence à ce document Montré dans la recherche
Type de fichier Champs Fichier(s) :
Organisme vivant modifié Organisme récepteur ou organismes parentaux 68
Organisme vivant modifié OVM connexe(s) 1
Décision de pays ou tout autre communication Identification de l'OVM 42
Évaluation des risques générée par un processus de réglementation Organisme(s) vivant(s) modifié(s) 40
Laboratoire pour la détection et d'identification des OVM OVM(s) détectable(s) par le laboratoire 33

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