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Organisme vivant modifié
(LMO)
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Roundup Ready™ cotton
EN
1445 (MON1445)
Oui
MON-Ø1445-2
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Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com,
Cotton line 1445 was genetically engineered to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into the cotton genome by Agrobacterium-mediated transformation.
EN
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
Variety: Coker 312
EN
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MON-89383-1 - Roundup Ready™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine), Résistance aux herbicides (Glyphosate)
PV-GHGT07
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- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Kanamycine)
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoteur
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminateur
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Streptomycine)
Information on the inserted DNA sequences
Molecular characterization demonstrates that one T-DNA insert was integrated into the cotton genome to produce cotton 1445. The insert contains the CoMV promoter region, a codon optimised cp4epsps coding sequence, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions.
Vector information
The plasmid vector PV-GHGT07 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
EN
Molecular characterization demonstrates that one T-DNA insert was integrated into the cotton genome to produce cotton 1445. The insert contains the CoMV promoter region, a codon optimised cp4epsps coding sequence, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions.
Vector information
The plasmid vector PV-GHGT07 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
FR
- Nourriture
- Nourriture pour les animaux
- MON-Ø1445-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-Ø1445-2 - CropLife International Detection Methods Database [ English ]
- MON-Ø1445-2 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-Ø1445-2 - CropLife International Detection Methods Database ( CropLife ) [ English ]
Coding sequences of the EPSPS gene was altered for optimal expression in plants.
EN
The EPSPS enzyme is present in all plants, bacteria, and fungi, and is part of an important biochemical pathway called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional cotton plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
EN
- Roundup Cotton APHIS [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 4 | |
| Organisme vivant modifié | OVM connexe(s) | 1 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 27 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 19 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 12 | |
