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Organisme vivant modifié
(LMO)
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Herbicide-tolerant, insect-resistant maize
EN
MIR162 × NK603 × DAS40278
Oui
SYN-IR162-4 × MON-ØØ6Ø3-6 × DAS-4Ø278-9
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Organisation :Syngenta ()Téléphone :Télécopieur :E-mail :Site Web : http://www.syngentaseeds.com/seedsmain.aspx,
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Organisation :Dow AgroSciences ()Téléphone :Télécopieur :E-mail :Site Web : http://www.dowagro.com/homepage/index.htm,
The maize (Zea mays) was produced through crossing modified parental lines for resistance to insect pests and tolerance to herbicides. For protection from Lepidoptera insects, the maize expresses VIP3Aa20 protein, which has a pore-forming mode of action that selectively damages the midgut epithelium line of feeding larvae. For tolerance to glyphosate, the maize expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate, which has a low binding affinity for the herbicidal compound and allows for the continued synthesis of aromatic amino acids through the shikimate pathway. For tolerance to aryloxyphenoxypropionate and 2,4-dichlorophenoxyacetic acid herbicides (acetyl‐CoA carboxylase inhibitors), the modified maize expresses Sphingobium herbicidovorans aryloxyalkanoate dioxygenase, which cleaves 2,4-dichlorophenoxyacetic acid into non-herbicidal dichlorophenol and glyoxylate, as well as inactivates aryloxyphenoxypropionate. In addition, the modified maize also contains a gene cassette for Escherichia coli phosphomannose isomerase, which was used as a selectable marker during transformation by allowing for the transformed maize plants to use mannose as a carbon source.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-246-6 Organisme Zea mays (Maize, Corn, MAIZE)Cultures
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BCH-LMO-SCBD-100885-13 Organisme vivant modifié SYN-IR162-4 - Agrisure™ Viptera maizeRésistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-LMO-SCBD-14776-18 Organisme vivant modifié MON-ØØ6Ø3-6 - Roundup Ready™ maizeMonsanto | Résistance aux herbicides (Glyphosate)
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BCH-LMO-SCBD-104814-2 Organisme vivant modifié DAS-4Ø278-9 - Enlist™ MaizeDow AgroSciences GmbH | Résistance aux herbicides, Tolerance to 2,4-Dichlorophenoxyacetic acid, Tolerance to aryloxyphenoxypropionate
FR
pNOV1300; PV-ZMGT32; pDAS1740
EN
- Croisement
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoteur
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BCH-GENE-SCBD-100887-5 Vegetative insecticidal protein 3Aa20 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminateur
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Séquence codante de protéines | Mannose tolerance,Gènes marqueurs et gènes rapporteurs sélectables
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-100364-5 Rice actin 1 gene promoter | Oryza sativa (Rice, ORYSA)Promoteur
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BCH-GENE-SCBD-100355-6 Rice actin 1, intron | Oryza sativa (Rice, ORYSA)Intron
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-104795-4 RB7 matrix attachment region | Nicotiana tabacum (Tobacco, TOBAC )Enhancer
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BCH-GENE-SCBD-104812-3 Aryloxyalkanoate dioxygenase gene | Sphingobium herbicidovorans (SPHHE)Séquence codante de protéines | Résistance aux herbicides,Tolerance to 2,4-Dichlorophenoxyacetic acid,Tolerance to aryloxyphenoxypropionate
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BCH-GENE-SCBD-104813-4 Per5 3' Untranslated Region | Zea mays (Maize, Corn, MAIZE)Terminateur
DNA insert from MIR162 (SYN-IR162-4) vector pNOV1300
The parental genome contains two gene cassettes: a variant of the native Bacillus thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20) and Escherichia coli phosphomannose isomerase.
Transcription of vip3Aa20 commences at the Zea mays ubiquitin promoter and then transcribes vip3Aa20 followed by intron 9 of Z. mays phosphoenolpyruvate carboxylase, before terminating at the Cauliflower mosaic virus 35S terminator. Higher levels of expression are expected due to the promoter and the enhancement by the intron.
The pmi coding sequence is under the control of Z. mays ubiquitin promoter and transcription terminates at the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator.
Note:
The parental genome contains two gene cassettes: a variant of the native Bacillus thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20) and Escherichia coli phosphomannose isomerase.
Transcription of vip3Aa20 commences at the Zea mays ubiquitin promoter and then transcribes vip3Aa20 followed by intron 9 of Z. mays phosphoenolpyruvate carboxylase, before terminating at the Cauliflower mosaic virus 35S terminator. Higher levels of expression are expected due to the promoter and the enhancement by the intron.
The pmi coding sequence is under the control of Z. mays ubiquitin promoter and transcription terminates at the Agrobacterium tumefaciens nopaline synthase gene (nos) terminator.
Note:
- Southern blot analyses demonstrated that the T-DNA insert contains: (i) single copies of a vip3Aa20 gene and a pmi gene; (ii) two copies of the maize ubiquitin promoter; (iii) one copy of the nos terminator; and (iv) no backbone sequences from transformation plasmid pNOV1300.
- In the parental MIR162 maize, a variant of the native B. thuringiensis vegetative insecticidal protein 3Aa (vip3Aa20), named vip3Aa19, which has codon changes that result in a single M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution.
DNA insert from NK603 (MON-ØØ6Ø3-6) vector PV-ZMGT32
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps). In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and an A. tumefaciens nopaline synthase gene (nos) terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by a Cauliflower mosaic virus enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the Zea mays heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promoter high levels of transcription.
Note:
The plant expression plasmid vector, PV-ZMGT32 contains two adjacent plant gene expression cassettes each containing a single copy of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps). In the first (5' end) expression cassette, the cp4-epsps gene is under the transcriptional regulation of an Oryza sativa actin promoter and an A. tumefaciens nopaline synthase gene (nos) terminator. An O. sativa actin intron is also present in the transcript for enhanced expression of the coding sequence. The second cassette consists of another cp4-epsps gene regulated by a Cauliflower mosaic virus enhanced 35S promoter (containing a duplicated enhancer region) and a nos terminator. Similarly, an intron from the Zea mays heat shock protein 70 (hsp70) was included for enhancing expression of the coding sequence. Both promoters of the gene cassettes are expected to promoter high levels of transcription.
Note:
- The parental NK603 line contained a single, intact insertion containing both cp4-epsps gene cassettes.
- Due to a restriction digest prior to particle bombardment, the vector backbone, containing Escherichia coli neomycin phosphotransferase II and origin of replication, were not incorporated into the parental genome.
DNA insert from DAS40278 (DAS-4Ø278-9) vector pDAS1740
The parental LMO was generated using the Whiskers-mediated transformation method. Sphingobium herbicidovorans aryloxyalkanoate dioxygenase-1 (aad-1) is under the control of Zea mays ubiquitin gene promoter and Z. mays root preferential cationic peroxidase terminator. Elevated levels of transcription are expected to occur due to the constitutive nature of the ubiquitin promoter.
Note:
- The aad-1 coding sequence was optimized for expression in the plant.
- Southern blot analysis indicated that a single complete copy of the transformation cassette was stably integrated into the host genome at a single locus.
- No integration of the vector backbone occurred.
For more information, kindly refer to the parental LMO record.
FR
- Nourriture pour les animaux
- Nourriture
- DAS-4Ø278-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR162-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR162-4 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
FR
- EUginius - MIR162 x NK603 x DAS40278 [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Décision de pays ou tout autre communication | Identification de l'OVM | 1 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 1 | |
