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Organisme vivant modifié
(LMO)
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Insect-resistant soybean
EN
MON94637
Oui
MON-94637-8
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Organisation :Bayer CropScience AG ()Alfred-Nobel-Strasse 50Monheim am Rhein,
D - 40789, AllemagneTéléphone :Télécopieur :E-mail :Site Web :
Soybean (Glycine max) was modified to confer resistance to the lepidopteran insect pests: soybean looper (Chrysodeixis includens) and velvetbean caterpillar (Anticarsia gemmatalis). For insect resistance the soybean expresses two chimeric cry genes derived from Bacillus thuringiensis, cry1A.2 and cry1B.2. The Cry proteins function as insecticidal toxins that, when ingested, are proteolytically activated in the midgut of the insects, Chrysodeixis includens and Anticarsia gemmatalis, and bind to specific receptors on epithelial cells, forming pores that lead to insect death.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-10453-8 Organisme Glycine max (Soybean, Soya bean, Soya / soja /соя, SOYBN)Cultures
Soybean cultivar A3555 mid−maturity group III developed by Asgrow Seed Company
EN
PV-GMIR527237
EN
- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-104802-5 Polyubiquitin10 gene promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoteur
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BCH-GENE-SCBD-280719-1 Cry1B.2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Ver de la capsule du coton (Helicoverpa spp.), Pyrale du maïs (Ostrinia nubilalis), Noctuelle ponctuée (Spodoptera frugiperda))
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BCH-GENE-SCBD-280718-1 Cry1A.2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites), Ver de la capsule du coton (Helicoverpa spp.), Pyrale du maïs (Ostrinia nubilalis), Noctuelle ponctuée (Spodoptera frugiperda))
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BCH-GENE-SCBD-280717-1 Lipoxygenase protein 3’ untranslated region | Medicago truncatula (Barrelclover, Strong-spined medick, Barrel medic, Barrel medick, MEDTR)Terminateur
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BCH-GENE-SCBD-280716-1 CCCH-Type zinc finger protein 3’ untranslated region | Medicago truncatula (Barrelclover, Strong-spined medick, Barrel medic, Barrel medick, MEDTR)Terminateur
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BCH-GENE-SCBD-280764-1 Chlorophyll a-b binding protein (CAB) promoter | Cucumis melo (Melon, Melons)Promoteur
Information about the inserted DNA sequences
The modified soybean contains two gene cassettes integrated into the soybean genome:
(1) The Cry1A.2 cassette contains the promoter, leader and intron from the polyubiquitin gene ubq10 of Arabidopsis thaliana, a codon optimized gene fusion comprised of sequences encoding Cry1Ah, Cry1Ac, and Cry1Ca domains from Bacillus thuringiensis, and the 3ʹ UTR sequence from Medicago truncatula (barrel medic) of a gene encoding a putative zinc finger protein, which directs polyadenylation of the mRNA.
(2) The Cry1B.2 cassette contains the promoter and leader of a chlorophyll a/b-binding (CAB) protein from Cucumis melo (melon), which directs the transcription; a codon optimized gene fusion comprised of sequences encoding Cry1Be, Cry1Ka2 and Cry1Ab domains from Bacillus thuringiensis, and the 3ʹ UTR sequence from a lipoxygnase gene of Medicago truncatula which directs polyadenylation of the mRNA .
Note:
The modified soybean contains two gene cassettes integrated into the soybean genome:
(1) The Cry1A.2 cassette contains the promoter, leader and intron from the polyubiquitin gene ubq10 of Arabidopsis thaliana, a codon optimized gene fusion comprised of sequences encoding Cry1Ah, Cry1Ac, and Cry1Ca domains from Bacillus thuringiensis, and the 3ʹ UTR sequence from Medicago truncatula (barrel medic) of a gene encoding a putative zinc finger protein, which directs polyadenylation of the mRNA.
(2) The Cry1B.2 cassette contains the promoter and leader of a chlorophyll a/b-binding (CAB) protein from Cucumis melo (melon), which directs the transcription; a codon optimized gene fusion comprised of sequences encoding Cry1Be, Cry1Ka2 and Cry1Ab domains from Bacillus thuringiensis, and the 3ʹ UTR sequence from a lipoxygnase gene of Medicago truncatula which directs polyadenylation of the mRNA .
Note:
- For more information regarding the transformation of the parental line, kindly refer to the 'Additional information section';
DNA sequence alignment of the event MON94637 and the PV-GMIR527237 transformation plasmid confirmed that the modified soybean genome does not contain any sequences from the plasmid vector;
- Both Cry1A.2 and Cry1B.2 proteins are expressed throughout the plant (except Cry1B.2 which was not detected in root tissue). Expression levels are low in grain;
- The novel chimeric cry1A.2 and cry1B.2 genes are codon-optimized for expression in soybean and are composed of multiple domains of existing Cry1 proteins derived from B. thuringiensis. Each domain was either synthetically synthesized or PCR-amplified from an existing vector. As a result, no other genes from Bacillus thuringiensis are carried over into the MON 94637 genome;
- Next generation sequence reads from MON94637 (R3) that mapped to the intended T-DNA I insert were analysed and two unique insert-flank junction sites were identified. Each comprised the inserted T-DNA I border sequence joined to a flanking sequence in the soybean genome. This indicates that a single copy of the T-DNA I insert has been integrated into the genome of MON94637.
FR
- Nourriture
- MON-94637-8 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
To create the MON94637 soybean line, the conventional soybean variety A3555 was transformed using the binary plasmid vector PV-GMIR527237. This plasmid contains two separate transfer DNAs (T-DNAs):
(1) T-DNA I – contains the two trait expression cassettes;
(2) T-DNA II – contains the selectable marker cassettes splA and aadA. The aadA gene encodes an aminoglycoside modifying enzyme conferring spectinomycin and streptomycin resistance. The splA gene encodes a sucrose phosphorylase, which interferes with the sucrose metabolism during embryo development by converting sucrose into fructose and glucose–1–phosphate resulting in a recognizable wrinkled seed phenotype.
While both T-DNAs were inserted into the soybean genome during transformation, plants that contained only T-DNA I, and not T-DNA II, were isolated for further development.
(1) T-DNA I – contains the two trait expression cassettes;
(2) T-DNA II – contains the selectable marker cassettes splA and aadA. The aadA gene encodes an aminoglycoside modifying enzyme conferring spectinomycin and streptomycin resistance. The splA gene encodes a sucrose phosphorylase, which interferes with the sucrose metabolism during embryo development by converting sucrose into fructose and glucose–1–phosphate resulting in a recognizable wrinkled seed phenotype.
While both T-DNAs were inserted into the soybean genome during transformation, plants that contained only T-DNA I, and not T-DNA II, were isolated for further development.
FR
- Euginius: MON94637 soybean detailed information (MON-94637-8) [URL] [ English ]
- OECD Biotrack database: MON-94637-8 [URL] [ English ]
- FSANZ: A1310 - Food derived from insect-protected soybean line MON94637 [URL] [ English ]
- FSANZ: A1310 Safety Assessment - Food derived from insect-protected soybean line MON94637 [PDF] ( Food Standards Australia New Zealand ) [ English ]
- ISAAA GM approval database: Crop event MON 94637 (International Service for the Acquisition of Agri-biotech Applications) [URL] [ English ]
| Type de fichier | Champs | Fichier(s) : | |
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| Décision de pays ou tout autre communication | Identification de l'OVM | 2 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 2 | |
