The modified potato contains three cassettes for Streptococcus pyogenes CRISPR-associated endonuclease 9 (cas9), guide RNAs targeting the endogenous potato genes StDMR6-1 and StCHL1, and Escherichia coli hygromycin phosphotransferase (hyg).

-The guide RNA expression cassette contains guide RNAs complementary to protospacer sequences in the endogenous potato genes StDMR6-1 and StCHL1 fused to a guide RNA scaffold. Expression of the guide RNAs is regulated by the Oryza sativa OsU3 RNA polymerase III promoter and a small (6pb) RNA transcription terminator (pol A III terminator).
-The cas9 coding sequence is under the control of the Cauliflower mosaic virus 35S promoter and the Agrobacterium tumefaciens nopaline synthase (NOS) terminator.
-The hyg coding sequence is used as a selectable marker during plant transformation and it is under the control of the Cauliflower mosaic virus 35S promoter  and terminator.

Note:
• According to information provided by the developer, foreign DNA sequences remain integrated in the genome of the modified line, including the hygromycin resistance marker gene.
• The pRGEB31 transformation vector additionally contains a kanamycin resistance gene used for bacterial selection and other cloning elements; however, information was not provided regarding the integration of vector backbone sequences into the potato genome.
• The binary vector pRGEB31 is not self-transmissible and requires the helper plasmid pRK2013 for transfer between bacterial cells. The helper plasmid was not present in the Agrobacterium tumefaciens strain used for transformation and is absent from the transformed potato genome.