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Organisme vivant modifié (LMO)
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Décisions sur l’OVM Évaluations des risques  
published: 10 août 2007 last updated: 08 févr. 2019
Identité de l’organisme vivant modifié
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Roundup Ready2Yield™ soybean
EN
MON89788
Oui
MON-89788-1
MON89788 is a second-generation glyphosate-tolerant soybean product, was developed by introduction of the cp4 epsps gene cassette consisting of chimeric promoter sequence derived from Arabidopsis thaliana Tsf1 gene promoter and enhancer sequences and from the 35S of the Figwort Mosaic Virus. In addition, the transformation was based on a new technique of Agrobacterium-mediated gene delivery to soybean meristem, where cells were induced directly to form shoots and give rise to transgenic plants
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Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
Variety: A3244
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Caractéristiques du processus de modification
PV-GMGOX20
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  • Transgénèse par agrobacterium
Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
  • BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoteur
  • BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Leader
  • BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Séquence signal
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminateur
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Leader
Information on the inserted DNA sequences:
The DNA inserted into the soybean genome contains:
- Codon optimized coding sequence of the aroA (epsps) gene from the Agrobacterium sp. strain CP4 encoding the CP4 EPSPS protein.
- a chimeric transcriptional promoter (P-FMV/Tsf1) consisting of chimeric sequence derived from Arabidopsis thaliana Tsf1 gene promoter and enhancer sequences from  the 35S of the Figwort Mosaic Virus. Located between the promoter and the cp4epsps coding sequence are the non-translated leader sequence (L-Tsf1) and the I-Tsf1 non translated intron;
- a chloroplast transit peptide sequence (TS-CTP2), derived from the Arabidopsis thaliana epsps gene and placed before the cp4 epsps gene encoding sequence in the gene insert;
- a polyadenylation sequence from RbcS2 gene (T-E9), derived from Pisum sativum containing the 3’ non translated region of the pea ribulose-1,5 biphosphate carboxylase small sub unit E9.

Vector information
The PV-GMGOX20 vector is approximately 9.7 kb and contains a cp4 epsps gene expression cassette delineated by left and right border regions. The T-DNA (transferred DNA) that is incorporated into the soybean genome is approximately 4.3 kb, and the DNA backbone region that is not incorporated into the soybean genome is approximately 5.4kb.

Southern blot analyses indicate that MON 89788 contains a single intact cp4 epsps expression cassette integrated at a single locus within the soybean genome. DNA sequencing analyses of the MON 89788 insert confirm the expected coding region of the cp4 epsps gene cassette, is identical to that transformed in the T-DNA cassette. No backbone sequences from the transformation plasmid were detected and no partial genetic elements, linked or unlinked to the inserted expression cassette were detected.
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Caractéristiques de l’OVM
FR
  • Nourriture
  • Nourriture pour les animaux
Informations supplémentaires
The soybean line MON98788 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option for soybean. This genetically engineered soybean variety contains a glyphosate tolerant form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) isolated from the common soil bacterium, Agrobacterium tumefaciens strain CP4 (CP4 EPSPS).

The EPSPS enzyme is part of the shikimate pathway that is involved in the production of aromatic amino acids and other aromatic compounds in plants. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to survive. This enzyme is present in all plants, bacteria, fungi, but not in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammalian, avian or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is normally present in food derived from plant and microbial sources.

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Les fichiers qui font référence à ce document Montré dans la recherche
Type de fichier Champs Fichier(s) :
Évaluation des risques générée par un processus de réglementation Organisme(s) vivant(s) modifié(s) 28
Décision de pays ou tout autre communication Identification de l'OVM 25
Laboratoire pour la détection et d'identification des OVM OVM(s) détectable(s) par le laboratoire 25
Organisme vivant modifié Organisme récepteur ou organismes parentaux 9
Organisme vivant modifié OVM connexe(s) 1