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Record information and status
Record ID
Date of creation
2012-05-08 20:48 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-08 20:48 UTC (dina.abdelhakim@cbd.int)

General Information
Comparison of tuber proteomes of potato varieties, landraces, and genetically modified lines
Satu J. Lehesranta, Howard V. Davies, Louise V.T. Shepherd, Naoise Nunan, Jim W. McNicol, Seppo Auriola, Kaisa M. Koistinen, Soile Suomalainen, Harri I. Kokko, and Sirpa O. Ka¨renlampi
Author’s contact information
Sirpa O. Karenlampi
Email sirpa.karenlampi@uku.fi;

Institute of Applied Biotechnology
University of Kuopio, FIN-70211 Kuopio, Finland
fax 358-17-163148.
  • English
Publication date
Summary, abstract or table of contents

Crop improvement by genetic modification remains controversial, one of the major issues being the potential for unintended effects. Comparative safety assessment includes targeted analysis of key nutrients and antinutritional factors, but broader scale-profiling or ''omics'' methods could increase the chances of detecting unintended effects. Comparative assessment should consider the extent of natural variation and not simply compare genetically modified (GM) lines and parental controls. In this study, potato (Solanum tuberosum) proteome diversity has been assessed using a range of diverse non-GM germplasm. In addition, a selection of GM potato lines was compared to assess the potential for unintended differences in protein profiles. Clear qualitative and quantitative differences were found in the protein patterns of the varieties and landraces examined, with 1,077 of 1,111 protein spots analyzed showing statistically significant differences. The diploid species Solanum phureja could be clearly differentiated from tetraploid (Solanum tuberosum) genotypes. Many of the proteins apparently contributing to genotype differentiation are involved in disease and defense responses, the glycolytic pathway, and sugar metabolism or protein targeting/storage. Only nine proteins out of 730 showed significant differences between GM lines and their controls. There was much less variation between GM lines and their non-GM controls compared with that found between different varieties and landraces. A number of proteins were identified by mass spectrometry and added to a potato tuber two-dimensional protein map
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
DOI: ​10.​1104/​pp.​105.​060152
Publisher and its location
American Society of Plant Biologists
© 2005 American Society of Plant Biologists
10 page PDF
Plant Physiology
Keywords and any other relevant information
Citation: Plant Physiology, July 2005, Vol. 138, pp. 1690-1699