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Record information and status
Record ID
Date of creation
2012-05-09 16:19 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-09 16:19 UTC (dina.abdelhakim@cbd.int)

General Information
Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion
Rita Batista, Nelson Saibo, Tiago Lourenc, and Maria Margarida Oliveira
Author’s contact information
Rita Batista
E-mail: rita.batista@insa.min-saude.pt

Instituto Nacional de Sau´ de Dr. Ricardo Jorge,
Avenida Padre Cruz, 1649-016 Lisbon,
  • English
Publication date
Summary, abstract or table of contents

Controversy regarding genetically modified (GM) plants and their potential impact on human health contrasts with the tacit acceptance of other plants that were also modified, but not considered as GM products (e.g., varieties raised through conventional breeding such as mutagenesis). What is beyond the phenotype of these improved plants? Should mutagenized plants be treated differently from transgenics? We have evaluated the extent of transcriptome modification occurring during rice improvement through transgenesis versus mutation breeding. We used oligonucleotide microarrays to analyze gene expression in four different pools of four types of rice plants and respective controls: (i) a γ-irradiated stable mutant, (ii) the M1 generation of a 100-Gy γ-irradiated plant, (iii) a stable transgenic plant obtained for production of an anticancer antibody, and (iv) the T1 generation of a transgenic plant produced aiming for abiotic stress improvement, and all of the unmodified original genotypes as controls. We found that the improvement of a plant variety through the acquisition of a new desired trait, using either mutagenesis or transgenesis, may cause stress and thus lead to an altered expression of untargeted genes. In all of the cases studied, the observed alteration was more extensive in mutagenized than in transgenic plants. We propose that the safety assessment of improved plant varieties should be carried out on a case-by-case basis and not simply restricted to foods obtained through genetic engineering.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
doi: 10.1073/pnas.0707881105
Publisher and its location
National Academy of Science of the United States of America
© 2008 by The National Academy of Sciences of the USA
6 page PDF
Proceedings of the National Academy of Sciences (PNAS)
Keywords and any other relevant information
Keywords: food safety evaluation, rice, genetically modified organisms, genetic engineering, gamma irradiation

Citation:PNAS March 4, 2008 vol. 105 no. 9 3640-3645