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Record information and status
Record ID
Date of creation
2012-05-09 18:08 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-09 18:08 UTC (dina.abdelhakim@cbd.int)

General Information
The stability of the Arabidopsis transcriptome in transgenic plants expressing the marker genes nptII and uidA.
Souad El Ouakfaoui and Brian Miki
Author’s contact information
Brian Miki
Email: mikib@agr.gc.ca

Bioproducts and Bioprocesses, Research Branch,
Agriculture and Agri-Food Canada,
Ottawa, ON K1A 0C6, Canada

fax: 613 759 1701
  • English
Publication date
Summary, abstract or table of contents

The ATH1 Arabidopsis GeneChip from Affymetrix was used to search for transcriptome changes in Arabidopsis associated with the strong expression of transgenes regulated by constitutive promoters. The insertion and expression of the commonly used marker genes, uidA and nptII, did not induce changes to the expression patterns of the approximately 24 000 genes that were screened under optimal growth conditions and under physiological stress imposed by low temperatures. Approximately 8000 genes (35% of the Arabidopsis genome) underwent changes in gene expression in both wild-type and transgenic plants under abiotic stresses such as salt, dehydration, cold, and heat. This study provides detailed information on the extent of nontargeted or pleiotropic effects of transgenes on plants and shows that the transgenic and non-transgenic plants were equivalent in their global patterns of transcription. This information may help to extend our understanding and interpretation of the principle of substantial equivalence which is used as a first step in the biosafety evaluation of transgenic crops.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
doi: 10.1111/j.1365-313X.2005.02350.x
Publisher and its location
© Minister of Public Works and Government Services Canada
10 Page PDF
The Plant Journal
Keywords and any other relevant information
Keywords: Arabidopsis, pleiotropic effects, stress response, substantial equivalence, transgene, transcriptome, microarray analysis.

Citation: The Plant Journal (2005) 41, 791-800