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Record information and status
Record ID
Date of creation
2012-05-09 18:59 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-09 18:59 UTC (dina.abdelhakim@cbd.int)

General Information
Selectable marker genes and unintended changes to the plant transcriptome
Brian Miki, Ashraf Abdeen, Yuzuki Manabe and Phil MacDonald
Author’s contact information
Brian Miki
Email: mikib@agr.gc.ca

Eastern Cereals and Oilseeds Research Centre,
Agriculture and Agri-Food Canada,
Ottawa, ON, Canada, K1A 0C6

Fax: 613 7591701
  • English
Publication date
Summary, abstract or table of contents

The intended effect of a selectable marker gene is to confer a novel trait that allows for the selection and recovery of transgenic plants. Unintended effects may also occur as a result of interactions between the selectable marker gene or its regulatory elements and genetic elements at the site of insertion. These are called position effects. Other unintended effects may occur if the selectable marker gene has a range of pleiotropic effects related to the functional and regulatory domains within the coding region or the regulatory elements used to drive expression. Both pleiotropic and position effects may generate unpredictable events depending on the process used for transgenesis and the state of knowledge associated with the selectable marker gene. Although some selectable marker genes, such as the neomycin phosphotransferase type II gene ( nptII ), have no pleiotropic effects on the transcriptomes of transgenic plants, others, such as the bialaphos resistance gene (bar), have pleiotropic effects. These must be clearly understood and accounted for when evaluating the expression patterns conferred by other co-transforming transgenes under study. The number and kinds of selectable marker genes are large. A detailed understanding of their unintended effects is needed to develop transgenic strategies that will minimize or eliminate unintended and unpredictable changes to plants with newly inserted genes
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
  • Report / Review / Fact sheet / Notes
doi: 10.1111/j.1467-7652.2009.00400.x
Publisher and its location
Blackwell Publishing Ltd
© 2009 The Authors
8 page PDF
Keywords and any other relevant information
Keywords: phenotype, pleiotropic effects, position effects, selectable marker genes, transcriptome,transgenic plants, unintended effects.

Citation: Plant Biotechnology Journal(2009)7, pp. 211-218