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Record information and status
Record ID
Date of creation
2012-05-10 15:26 UTC (dina.abdelhakim@cbd.int)
Date of last update
2012-05-14 19:31 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-14 19:31 UTC (dina.abdelhakim@cbd.int)

General Information
Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: The case of a virus-resistant tomato
Davide Corpillo, Giulia Gardini, Anna Maria Vaira, Manuela Basso, Silvio Aime, Gian Paolo Accotto, Mauro Fasano
Author’s contact information
Mauro Fasano
Email: mauro.fasano@uninsubria.it

Dipartimento di Biologia Strutturale e Funzionale,
Università dell'Insubria, Varese, Italy
  • English
Publication date
Summary, abstract or table of contents

At present, the so-called "substantial equivalence" is the only widely accepted criterion for deciding whether or not a transgenic food is, from an alimentary point of view, to be considered totally correspondent to the "traditional" one from which it derives. Although never exactly defined, it deals with a comparison between the chemical composition of the two foods. A more in-depth analysis can be performed by one of the most suitable methods that allows for the simultaneous screening of many components without prior identification, the analysis of the proteome. As a model for testing this kind of approach, we compared protein expression of two types of tomato plants, having the same genetic background, except for a virus resistance trait introduced by genetic engineering. When proteins extracted from seedlings of the two types were analyzed by two-dimensional electrophoresis, no significant differences, either qualitative or quantitative, were detected, indicating that in this case the expression of major proteins was unmodified by the genetic manipulation. Fifteen proteins were identified by peptide mass fingerprinting.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
    • Environmental Monitoring
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
DOI: 10.1002/pmic.200300540
Publisher and its location
John Wiley and Sons
Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords and any other relevant information
Keywords: Genetically modified organism;Lycopersicon esculentum;Substantial equivalence;Tomato spotted wilt virus

Citation: PROTEOMICS Volume 4, Issue 1, pages 193-200, January 2004

Only Abstract Available