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Biosafety Virtual Library Resources
(VLR)
last updated: 10 May 2012
Gene stacking in transgenic plants – the challenge for 21st century plant biotechnology
EN
John Wiley and Sons
EN
2005
EN
Plant Biotechnology Journal
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© 2005, John Wiley and Sons
EN
Summary:
One of the major technical hurdles impeding the advance of plant genetic engineering and biotechnology is the fact that the expression or manipulation of multiple genes in plants is still difficult to achieve. Although a small proportion of commercial genetically modified (GM) crops present ‘stacked’ or ‘pyramided’ traits, only a handful of products have been developed by introducing three or more novel genes. On the research front, a variety of conventional and more novel methods have been employed to introduce multiple genes into plants, but all techniques suffer from certain drawbacks. In this review, the potential and problems of these various techniques and strategies are discussed, and the prospects for improving these technologies in the future are presented.
EN
One of the major technical hurdles impeding the advance of plant genetic engineering and biotechnology is the fact that the expression or manipulation of multiple genes in plants is still difficult to achieve. Although a small proportion of commercial genetically modified (GM) crops present ‘stacked’ or ‘pyramided’ traits, only a handful of products have been developed by introducing three or more novel genes. On the research front, a variety of conventional and more novel methods have been employed to introduce multiple genes into plants, but all techniques suffer from certain drawbacks. In this review, the potential and problems of these various techniques and strategies are discussed, and the prospects for improving these technologies in the future are presented.
- GBF-T17. Strengthen biosafety and distribute benefits of biotechnology
No
No
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Identifier (ISBN, ISSN, etc.)
doi: 10.1111/j.1467-7652.2004.00113.x
Format
15 page PDF
Keywords and any other relevant information
Keywords: chloroplast transformation, co-transformation, linked transgenes, marker gene excision, metabolic engineering, multigene manipulation, polyproteins, pyramiding, retransformation, stacking.
Citation: Plant Biotechnology Journal Volume 3, Issue 2, pages 141–155, March 2005
