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Record information and status
Record ID
Date of creation
2012-05-10 21:12 UTC (dina.abdelhakim@cbd.int)
Date of last update
2013-10-10 19:39 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-10-10 19:39 UTC (dina.abdelhakim@cbd.int)

General Information
Detection of RNA variants transcribed from the transgene in Roundup Ready soybean
Andreas Rang, Bettina Linke and Bärbel Jansen
Author’s contact information
Andreas Rang
e-mail: andreas.rang@charite.de

Federal Institute for Risk Assessment,
Center for Novel Foods and Genetic Engineering,
Thielallee 88-92, 14195 Berlin, Germany
  • English
Publication date
Summary, abstract or table of contents

The acreage for genetically modified crops (GMOs)-particularly soybean-has steadily increased since 1996, when the first crop of Roundup Ready soybean (intended for food production) was grown. The Roundup Ready soybean varieties derive from a soybean line into which a glyphosate-resistant enolpyruvylshikimate-3-phosphate-synthase (EPSPS) gene was introduced. The inserted and the flanking regions in Roundup Ready soybean have recently been characterized. It was shown that a further 250-bp fragment of the epsps gene is localized downstream of the introduced nos terminator of transcription, derived from the nopaline synthase gene from Agrobacterium tumefaciens. We examined whether this 250-bp fragment could be of functional importance. Our data demonstrate that at least 150 bp of this DNA region are transcribed in Roundup Ready soybean. Transcription of the fragment depends on whether read-through events ignore the nos terminator signal located upstream. Our data also indicate that the read-through product is further processed, resulting in four different RNA variants from which the transcribed region of the nos terminator is completely deleted. Deletion results in the generation of open reading frames which might code for (as yet unknown) EPSPS fusion proteins. The nos terminator is used as a regulatory element in several other GMOs used for food production. This implies that read through products and transcription of RNA variants might be a common feature in these GMOs.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
    • Detection
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
  • 3. Risk assessment of living modified plants with tolerance to abiotic stress
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
DOI: 10.1007/s00217-004-1064-5
Publisher and its location
© 2005, Springer Berlin / Heidelberg
6 page PDF
European Food Research and Technology
Keywords and any other relevant information
Keywords  Genetically modified organism (GMO) - Roundup Ready soybean - Termination of transcription - Post-transcriptional processing of RNA

European Food Research and Technology, 2005, Volume 220, Numbers 3-4, Pages 438-443