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Record information and status
Record ID
Date of creation
2012-05-11 19:40 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-05-11 19:40 UTC (dina.abdelhakim@cbd.int)

General Information
A Killer-Rescue system for self-limiting gene drive of anti-pathogen constructs
Fred Gould, Yunxin Huang, Mathieu Legros and Alun L Lloyd
Author’s contact information
Fred Gould
Email: fred_gould@ncsu.edu

North Carolina State University,
Raleigh, NC 27695, USA
  • English
Publication date
Summary, abstract or table of contents

A number of genetic mechanisms have been suggested for driving anti-pathogen genes into natural populations. Each of these mechanisms requires complex genetic engineering, and most are theoretically expected to permanently spread throughout the target species' geographical range. In the near term, risk issues and technical limits of molecular methods could delay the development and use of these mechanisms. We propose a gene-drive mechanism that can be self-limiting over time and space, and is simpler to build. This mechanism involves one gene that codes for toxicity (killer) and a second that confers immunity to the toxic effects (rescue). We use population-genetic models to explore cases with one or two independent insertions of the killer gene and one insertion of the rescue gene. We vary the dominance and penetrance of gene action, as well as the magnitude of fitness costs. Even with the fitness costs of 10 per cent for each gene, the proportion of mosquitoes expected to transmit the pathogen decreases below 5 per cent for over 40 generations after one 2 : 1 release (engineered : wild) or after four 1 : 2 releases. Both the killer and rescue genes will be lost from the population over time, if the rescue construct has any associated fitness cost. Molecular approaches for constructing strains are discussed.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
  • 5. Risk assessment of living modified mosquitoes species that act as vectors of human and animal diseases
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
doi: 10.1098/rspb.2008.0846
Publisher and its location
The Royal Society
Copyright © 2008 The Royal Society
8 page PDF
Proceedings of the Royal Society B
Keywords and any other relevant information
Keywords: gene drive; selfish genes; Aedes; Anopheles; dengue; malaria

Citation: Proc. R. Soc. B (2008) 275, 2823-2829