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Record information and status
Record ID
Date of creation
2012-08-02 14:29 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-02 14:29 UTC (dina.abdelhakim@cbd.int)

General Information
Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal.
Vijay Rani and S. N. Raina
Author’s contact information
S. N. Raina

Laboratory of Molecular and Cellular Cytogenics,
Department of Botany
University of Delhi
Delhi 110007, India

Email: snraina@satyam.net.in
  • English
Publication date
Summary, abstract or table of contents

The commercial multiplication of a large number of diverse plant species represents one of the major success stories of urilizing tissue culture technology profitably. Micropropagation has now become a multibillion dollar industry, practised all over the world. Of the various methods used to micropropagate plants, somatic embryogenesis and enhanced axillary branching have become the principal methods of multiplication. Long-term benefits of this enterprise, however, lie in the production of clonally uniform plants. The concept of genetic uniformity among micropropagated plants derived through organized meristems was exploded by several convincing reports of the incidence of somaclonal variation at morphological, cytological (chromosome number and structure), cytochemical (genome size), biochemical (proteins and isozymes), and molecular (nuclear and organellar genomes) levels. Somaclonal variation is not limited to any particular group of plants; it has been reported, for example, in ornamentals, plantation crops, vegetable and food crops, forest species and fruit trees. The upsurge of these reports, facilitated to a large extent by the technical developments made in molecular biology, is a matter of great concern for any micropropagation system. The economic consequences of somaclonal variation can be enormous in forest trees and woody plants, as they have long life cycles. Therefore, somaclonal variation has to be dispensed with if large-scale micropropagation of diverse plant species is to become not only successful but also accepted by end-users. In the light of the various factors (genotype, ploidy level, in vitro culture age, explant and culture type, etc.) that lead to somaclonal variation of divergent genetic changes at the cellular and molecular levels, genetic analysis of micropropagated plants using a multidisciplinary approach, especially at the DNA sequence level, initially and at various cultural stages, is essential. The results obtained at early multiplication stages from these tests could help in modifying the protocol/s for obtaining genetically true-to-type plants, and ultimate usage by entrepneneurs without any ambiguity.
Thematic areas
  • Scientific and technical issues
    • Risk assessment
Background material to the “Guidance on risk assessment of living modified organisms”
Is this document is recommend as background material for the “Guidance on Risk Assessment of Living Modified Organisms”
Section(s) of the “Guidance on Risk Assessment of Living Modified Organisms” this background material is relevant
Additional Information
Type of resource
  • Article (journal / magazine / newspaper)
DOI: 10.1007/s11627-000-0059-6
Publisher and its location
Copyright © 2000, Springer Berlin / Heidelberg
In Vitro Cellular and Development Biology - Plant, Volume 36, Number 5, September 2000 , pp. 319-330(12)
Keywords and any other relevant information
Key words  enhanced axillary branching - genetic analysis - micropropagation - somaclonal variation - somatic embryogenesis