DAS-21Ø23-5 × DAS-24236-5 × MON-88913-8 × SYN-IR1Ø2-7 - Widestrike™ x Roundup Ready Flex™ x VIPCOT™ Cotton | BCH-LMO-SCBD-105698 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
last updated: 25 Jul 2014
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Widestrike™ x Roundup Ready Flex™ x VIPCOT™ Cotton
EN
3006-210-23 x 281-24-236 x MON88913 x COT102
Yes
DAS-21Ø23-5 × DAS-24236-5 × MON-88913-8 × SYN-IR1Ø2-7
The stacked cotton line 3006-210-23 x 281-24-236 x MON88913 x COT102 was produced by cross breeding between each of the parental organisms to resist the lepidopteran pests such as the cotton bollworm (Helicoverpa zea), amongst others, as well as tolerance against the herbicide glyphosate and glufosate via the introduction of the Cry1Ac, Cry1F, Vip3A, PAT and EPSPS genes respectively.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
PV-GHGT35, pCOT-1, pMYC3006 and pAGM281
EN
  • Cross breeding
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)
    Promoter
  • BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)
    Intron
  • BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)
    Protein coding sequence | Resistance to herbicides (Glufosinate)
  • BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-103900-3 4ocs∆Mas2' promoter
    Promoter
  • BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Leader
  • BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Leader
  • BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
  • BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)
    Leader
  • BCH-GENE-SCBD-103907-3 Actin 8 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-103908-4 Actin 8 Leader sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Leader sequence
  • BCH-GENE-SCBD-103909-3 Actin 8 Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Intron
  • BCH-GENE-SCBD-104517-2 Actin 2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-14990-5 Vegetative insecticidal protein 3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)
    Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-101874-2 Ubiquitin gene 3 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Promoter
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
DNA insert from COT102 vector pCOT-1:
Cotton tolerant to lepidopteran pests through introduction of the vip3A(a) gene which codes for an insecticidal protein that targets lepidopteran insect species. The aph4 gene, coding for hygromycin-B phosphotransferase (APH4) was used as a selectable marker.

DNA insert from DAS-21Ø23-5, vector pMYC3006: 
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1Ac gene from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki strain HD73. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from DAS-24236-5, vector pAGM281:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from 88913 vector PV-GHGT35:
This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate.

For additional information on this LMO, please refer to the records of the parental LMOs.
EN
LMO characteristics
EN
  • Food
  • Fiber/textile
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 3
Risk Assessment generated by a regulatory process Living modified organism(s) 3