Rubber tree modified to express Human atrial natriuretic factor | BCH-LMO-SCBD-108909 | Living Modified Organism | Biosafety Clearing-House
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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 18 Sep 2015
Living Modified Organism identity
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Rubber tree modified to express Human atrial natriuretic factor
EN
HT3
No
Hevea brasiliensis was modified to express the Human atrial natriuretic factor (HANF), which s a peptide hormone that has been shown to lower cardiac blood pressure, particularly during open heart surgery. Expression of the HANF gene in Hevea brasiliensis allows for the commercial production of this protein for pharmaceutical purposes.

Hevea expressing HANF also harbours nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Clone GL1
EN
Characteristics of the modification process
pGPTV-HANF-HevP
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-108282-1 Atrial natriuretic factor coding sequence | Homo sapiens (HUMAN)
    Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal)
  • BCH-GENE-SCBD-108907-1 Hevien gene promoter | Hevea brasiliensis (Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
Four vector constructs were synthesized each containing a variable length of the heaven gene promoter (pGPTV-HANF-HevP1, pGPTV-HANF-HevP2, pGPTV-HANF-HevP3, and pGPTV-HANF-HevP4) each of which was placed upstream of the HANF coding sequence. 

The HANF and nptII genetic elements were placed in an antisense orientation relative to each other within the plasmid.

The inserted genetic material is the region between the left boarder and the right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
EN
LMO characteristics
EN
  • Pharmaceutical
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Country's Decision or any other Communication Living modified organism(s) 1

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