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Gene and DNA Sequence
Record information and status
Record ID
114695
Status
Published
Date of creation
2019-05-15 14:41 UTC (austein.mcloughlin@cbd.int)
Date of last update
2019-05-15 18:35 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-05-15 18:35 UTC (austein.mcloughlin@cbd.int)

General information
Name of the gene or DNA sequence
Arabidopsis thaliana and Nicotania tabacum chimeric Rubisco activase
Abbreviation
At-Nt RCase
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Nicotiana tabacum - Tobacco, TOBAC
Donor organism point of collection
NOTE: This protein contains coding sequences from Arabidopsis thaliana and Nicotiana tabacum.
Characteristics of the protein coding sequence
Biological function
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubsico) activase (RCase) facilitates activation of and the maintenance of Rubisco activity by using ATP hydrolysis to release tightly bound sugar phosphates from the catalytic sites of Rubisco.

Tobacco (N. tabacum) RCase is known for being heat stable, thus the chimeric protein was created to confer thermostability to the protein. A domain consisting of residues 267-334 in tobacco RCase were replaced by the corresponding Arabidopsis domain. Previously, overexpression of this chimeric protein promoted better growth and higher rates of photosynthesis under high temperatures.
Related trait(s) or use(s) in biotechnology
  • Increased photosynthetic rate

Records referencing this document (1)
IDDescription
1record(s) found
Modified Organism1 record