Arabidopsis thaliana and Nicotiania tabacum chimeric Rubisco activase | BCH-GENE-SCBD-114695 | Genetic element | Biosafety Clearing-House


Genetic element (GENE)

published: 15 May 2019 last updated: 11 Nov 2020
General information
Arabidopsis thaliana and Nicotiania tabacum chimeric Rubisco activase
At-Nt RCase
Protein coding sequence
Donor organism
NOTE: This protein contains coding sequences from Arabidopsis thaliana and Nicotiana tabacum.
Characteristics of the protein coding sequence
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubsico) activase (RCase) facilitates activation of and the maintenance of Rubisco activity by using ATP hydrolysis to release tightly bound sugar phosphates from the catalytic sites of Rubisco.

Tobacco (N. tabacum) RCase is known for being heat stable, thus the chimeric protein was created to confer thermostability to the protein. A domain consisting of residues 267–334 in tobacco RCase were replaced by the corresponding Arabidopsis domain. Previously, overexpression of this chimeric protein promoted better growth and higher rates of photosynthesis under high temperatures.
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Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 1
Living Modified Organism Genetic elements construct 1