MON-Ø4Ø32-6 - Roundup Ready™ soybean | BCH-LMO-SCBD-14796 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 05 Jun 2006 last updated: 15 Dec 2021
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ soybean
GTS 40-3-2 (40-3-2)
The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate. The EPSPS gene put into GTS 40-3-2 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens called CP4 and the form of EPSPS enzyme produced by this gene is tolerant to glyphosate.  
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Line: A5403
Characteristics of the modification process
  • Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
  • BCH-GENE-SCBD-103899-3 Chloroplast Transit Peptide 4 | Petunia hybrida (Petunia, PETHY)
    Transit signal
The plasmid PV-GMGT04 contained three transformation cassettes driven by plant promoters: two Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) genes and a gene encoding ß-glucuronidase (GUS) from Escherichia coli. Only a portion of  this vector was incorporated into event 40-3-2. Southern blot and PCR analysis indicated that only a single transformation cassette containing the EPSPS coding sequence was integrated into the host genome. The largest insert, containing the function CP4 EPSPS gene, contained a deletion in the enhancer region of the E35S promoter and the remainder of the E35S promoter was functional. Analysis also indicated that there was no integration of segments of the vector backbone, the GUS coding sequence or the CmoVb promoter.

The cp4-epsps coding sequence encodes a 455 amino acid protein is terminated by tandem stop codons, and results in the synthesis of the full length and functional  ~46kDa CP4EPSPS protein in Round-up Ready soybean event 40-3-2 as confirmed by western blotting, enzyme-linked immunosorbent assay and EPSPS enzyme activity assays.

LMO characteristics
  • Feed
  • Food
Additional Information
The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, that is involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammals, birds or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.