MON-718ØØ-4 - Roundup Ready™ wheat | BCH-LMO-SCBD-45398 | Living Modified Organism | Biosafety Clearing-House


Living Modified Organism (LMO)

Decisions on the LMO Risk Assessments  
published: 11 Jun 2008 last updated: 26 Jan 2015
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ wheat
MON71800 wheat was modified to introduce the EPSPS gene, isolated from Agrobacterium tumefaciens strain CP4, to confer tolerance to the herbicide glyphosate.

NOTE: This LMO was formerly referred to with the MON-718ØØ-3.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Spring wheat variety ‘Bobwhite’
Characteristics of the modification process
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Wheat MON71800 contains two copies of cp4 epsps derived from the bacterium Agrobacterium tumefaciens sp. strain CP4.
LMO characteristics
  • Food
  • Feed
Detection method(s)
Additional Information
Roundup Ready® wheat (MON 71800) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in spring wheat production. This genetically engineered spring wheat contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 71800 to survive an otherwise lethal application of glyphosate. The EPSPS gene introduced into MON 71800 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4, and the novel form of the EPSPS enzyme produced by this gene is tolerant to glyphosate.

The EPSPS enzyme is part of the shikimate pathway, an important biochemical pathway in plants involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed for growth and survival. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, since these organisms are unable to synthesize their own aromatic amino acids. Because the aromatic amino acid pathway is not present in mammals, birds, or aquatic life forms, glyphosate has little, if any, toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources. MON 71800 was developed by introducing two CP4 EPSPS genes into the spring wheat variety ‘Bobwhite’ using Agrobacterium-mediated transformation.

The food and livestock safety of MON 71800 wheat was based on the safety assessment of the CP4 EPSPS protein and the level of expression of the protein in the grain. The CP4 EPSPS proteins constitutes a small amount of the total protein in MON 71800 so there is little dietary exposure. The lack of toxicity or allergenicity of CP4 EPSPS was demonstrated from the results of laboratory and safety studies. The nutritional equivalence and wholesomeness of MON 71800 wheat compared to conventional wheat was demonstrated by the analysis of key nutrients in the grain including proximates (e.g., crude protein, crude fat, crude fibre, ash, moisture), total dietary fibre, sugars, starch, amino acid and fatty acid composition, B vitamins and vitamin E, minerals, as well the composition in the anti-nutrient phytic acid.
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