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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Pea modified for increased protein content
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Lines 14/10 and 14/3
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- Organization: Institute for Plant Genetics and Crop Plant Research (IPK-Gatersleben) () | BCH-CON-SCBD-49240-1Organization:Institute for Plant Genetics and Crop Plant Research (IPK-Gatersleben) ()Corrensstrasse 3 Gatersleben D-06466 Germany,
,Phone:Fax:Email:Website: www.ipk-gatersleben.de,
The Vfaap1 gene from Vicia faba encodes an amino acid permease. Amino acid permeases transport amino acids from the plant vascular tissue into the symplasts of plant cells. They are differentially expressed according to plant development and tissue, and exhibit a range of specificity patterns for amino acids. VfAAP1 mediates the transport of a range of amino acids (mainly cysteine, arginine, glutamine, serine, leucine, methionine, histidine, glycine and threonine) with particular emphasis on cysteine. The formation of the amino acid permease of the field bean in pea seeds and the results in an increase in the seed protein content.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-100289-5 Organism Pisum sativum (Garden pea, PEA)Crops
Cultivar/ Breeding line: Eiffel
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pPZP200/VfAAP1 and pCAMBIA3300D
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-48368-4 Amino acid permease 1 gene | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Protein coding sequence | Changes in quality and/or metabolite content (Protein and amino acids)
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BCH-GENE-SCBD-100266-3 Legumin B4 gene promoter | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Promoter
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Notes about the Transcriptional regulator(s) specific to this LMO
The legumin B4 gene (leB4 gene) promoter from Vicia faba regulates the seed-specific expression of genes under its control.
Notes about the Other(s) sequence(s) specific to this LMO
The genetically modified peas were developed applying a co-transformation approach with the gene of interest (Vfaap1-gene) and the marker gene for selection of the transformed plant tissue (bar-gene) being located on two different plasmids pPZP200/VfAAP1 and pCAMBIA3300D respectively. As a result of the integration of the T-DNAs of both transformation plasmids at different locations in the plant genome and as a result of the breeding steps taken following regeneration of the fertile plants, the bar gene is no longer contained in the plants intended for release.
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The legumin B4 gene (leB4 gene) promoter from Vicia faba regulates the seed-specific expression of genes under its control.
Notes about the Other(s) sequence(s) specific to this LMO
The genetically modified peas were developed applying a co-transformation approach with the gene of interest (Vfaap1-gene) and the marker gene for selection of the transformed plant tissue (bar-gene) being located on two different plasmids pPZP200/VfAAP1 and pCAMBIA3300D respectively. As a result of the integration of the T-DNAs of both transformation plasmids at different locations in the plant genome and as a result of the breeding steps taken following regeneration of the fertile plants, the bar gene is no longer contained in the plants intended for release.
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- Feed
- Research
Expression of the Vfaap1 gene is controlled by the promoter of the legumin B4 gene (leB4 gene) from Vicia faba. This promoter regulates the seed-specific expression of the genes under its control. Expression in pollen has also been observed. In immature seeds of the transgenic pea line proposed for release, expression of the gene was verified by Northern blot. Other parts of the plant have not yet been examined for expression.
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In greenhouse studies on the genetically modified pea line 14/10 no differences in habitus (growth and flowering time) were found in comparison to the control line 14/3. The number and weight of the seeds and their protein content as well as the C:N ratio were also defined. With the exception of an increase in protein content, the genetically modified plants displayed normal phenotype.
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