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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Poplar modified for increased glutathione content
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ggs11
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Organization:University of Freiburg (ALU)Academic or research institute, Institut für Forstbotanik und Baumphysiologie [Institute of Forest Botany and Tree Physiology]Chair of Tree Physiology Georges-Köhler-Allee 53/54Freiburg,
79110, GermanyPhone: +49 761 203-8300,Fax: +49 761 203-8302,
Populus x canescens was transformed with the E.coli γ-glutamylcysteine synthetase gene resulting in the increased synthesis of glutathione in the cytosol of the plant's leaves. This was done to examine the oxidative stress tolerance capacity and possible detoxification capacity against various environmental pollutants exhibited by Populus x canescens expressing this modified genotype.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-101269-5 Organism Populus canescens (Gray poplar, Grey poplar, Hybrid poplar)Trees
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p70gshl, developed from pBIN19
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101271-4 Gamma-glutamylcysteine Synthetase I gene | Escherichia coli (ECOLX)Protein coding sequence | Use in industrial applications (Bioremediation)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
To transform the grey poplar the start codon of the endogenous gshI gene from E. coli was modified from TTG to ATG in the p70gshl construct. This causes an amino acid exchange from leucine to methionine.
Notes about the Other(s) sequence(s) specific to this LMO
The HindIII/SmaI fragment containing the gshI coding sequence (1.7 kb) was inserted into the same sites of the plasmid pLBR19, which contains the enhanced CaMV 35S promoter (P-e35S).
The P-e35S - gshI - T-35S cassette was cloned as an SstI/XbaI fragment into the binary vector pBIN 19 to create p70gshI.
Poplar line ggs11 possesses two copies of the DNA cassette.
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Notes about the Other(s) sequence(s) specific to this LMO
The HindIII/SmaI fragment containing the gshI coding sequence (1.7 kb) was inserted into the same sites of the plasmid pLBR19, which contains the enhanced CaMV 35S promoter (P-e35S).
The P-e35S - gshI - T-35S cassette was cloned as an SstI/XbaI fragment into the binary vector pBIN 19 to create p70gshI.
Poplar line ggs11 possesses two copies of the DNA cassette.
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- Timber
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For a detailed description of the modification of poplar line ggs11 see article by Arisi et al. (1997) below.
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- Arisi et al (1997) Planta 203, 362-372 [ English ]
- Gyulai et al (2008) Genetically Modified Plants, pp 173-191 [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
