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Living Modified Organism
(LMO)
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Modena potato with altered starch
EN
AV43-6-G7
Yes
AVE-436G7-1
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Person:BASFCarl-Bosch-Str. 64Limburgerhof,
67117, GermanyPhone: +4962160-0,Fax: +4962160-28117,Email: securemail@basf.com,Website:Related OrganizationBASF Plant Science GmbH ()Private sector (business and industry)Carl-Bosch-Str. 64Limburgerhof,
67117, GermanyPhone: +4962160-0,Fax: +4962160-28117,Email: securemail@basf.com,Website:
Potato was modified by the insertion of a fragment of the granule-bound starch synthase gene (gbss) in the anti-sense direction to down-regulate the endogenous gbss gene.
The anti-sense repeat fragment of the gbss gene leads to a reduction of the amylose fraction in the starch of the potato tuber resulting in a concomitant increase in the levels of amylopectine.
The potato event AVE436G7-1 does not contain any selective genes for resistance to antibiotics or herbicides.
EN
The anti-sense repeat fragment of the gbss gene leads to a reduction of the amylose fraction in the starch of the potato tuber resulting in a concomitant increase in the levels of amylopectine.
The potato event AVE436G7-1 does not contain any selective genes for resistance to antibiotics or herbicides.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar: Karnico
EN
pTiBo542ΔT
EN
- Agrobacterium-mediated DNA transfer
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0.850 kb
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1.211 kb
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1.339 kb
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1.211 kb
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0.253 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14997-6 Granule bound starch synthase gene promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-48072-3 Granule-bound starch synthase gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | altered carbohydrate composition: increased amylopectin content
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BCH-GENE-SCBD-45875-7 Beta-galactosidase gene | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
A truncated portion of the coding sequence of gbss gene (CS-gbss) was cloned in anti-sense orientation under control of the gbss gene promoter (P-gbss).
AVE-436G7-1 contains one insert of an inverted repeat construct of the potato gbss gene. This LMO also contains part of the beta-galactosidase gene (lac) gene from Escherichia coli. No elements outside of the left-border (LB) and right-border (RB) of the T-DNA were inserted. Both LB and RB sequences of the T-DNA were truncated during the insertion.
The portions of the GBSS gene sequence in the construct organization in this LMO is as follows:
- GBSS cDNA fragment; 5’ 1.1 kb part, Solanum tuberosum, sense orientation in relation to the promoter sequence.
- GBSS cDNA fragment; 3’ 1.3 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence, functioning as spacer element.
- GBSS cDNA fragment; 5’ 1.1 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence.
EN
AVE-436G7-1 contains one insert of an inverted repeat construct of the potato gbss gene. This LMO also contains part of the beta-galactosidase gene (lac) gene from Escherichia coli. No elements outside of the left-border (LB) and right-border (RB) of the T-DNA were inserted. Both LB and RB sequences of the T-DNA were truncated during the insertion.
The portions of the GBSS gene sequence in the construct organization in this LMO is as follows:
- GBSS cDNA fragment; 5’ 1.1 kb part, Solanum tuberosum, sense orientation in relation to the promoter sequence.
- GBSS cDNA fragment; 3’ 1.3 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence, functioning as spacer element.
- GBSS cDNA fragment; 5’ 1.1 kb part, Solanum tuberosum, antisense orientation in relation to the promoter sequence.
EN
- Food
- Feed
- Other (Industrial)
Southern blot showed one insertion site with one copy of the constructed was inserted into AVE-436G7-1. The insertion site in the recipient organism (potato variety "Karnico") in which the T-DNA was integrated in AVE-436G7-1 was found to have a deletion of 1814 bp.
EN
EN
- SNIF database [ English ]
- Czech Biosafety Clearing-House [ English ]
- av43-6-g7_application_food_feed_cultivation.pdf [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Laboratory for detection and identification of LMOs | LMO(s) detectable by the laboratory | 2 | |
| Living Modified Organism | Related LMO(s) | 2 | |
