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Living Modified Organism
(LMO)
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Poplar with modified lignin
EN
WT52-3 and WT52-40
No
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Person:Prof.dr. Wout Boerjan, Departement PlantensysteembiologieTechnologiepark 927Gent,
B-9052, BelgiumPhone: +32 9 331 38 81,Fax:Email: wout.boerjan@psb.vib-ugent.be,Related OrganizationVIB-UGent ()Academic or research instituteTechnologiepark 927Gent,
B-9052, BelgiumPhone: +32 9 331 38 81,Fax:Email: wout.boerjan@psb.vib-ugent.be,
The genetically modified poplar exhibits modified lignin (a major constituent of wood) due to the decreased activity of an enzyme of the lignin biosynthetic pathway. The altered enzyme is CCR (Cinnamoyl coenzymeA reductase). The down-regulation has been obtained by co-suppression. The enzyme residual activity varies between 3 to 100 % and is not necessarily uniform within the plant. Consequently, the quality or/and quantity of lignin is modified.
These modifications and the consequences on some wood properties have been described in several publications (Baucher et al., 1996, van Doorsselaere et al., 1995 ; Meyermans et al., 2000 ; Lapierre et al., 1999 ; Pilate et al., 2002 ; Lapierre et al., 2004).
In addition, the transgenic lines have also integrated the hygromycin phosphotransferase (hpt) gene that confers resistance to the antibiotic hygromycin. This antibiotic resistance is needed during in vitro culture steps to select cells that have been genetically modified from those that have not been modified.
EN
These modifications and the consequences on some wood properties have been described in several publications (Baucher et al., 1996, van Doorsselaere et al., 1995 ; Meyermans et al., 2000 ; Lapierre et al., 1999 ; Pilate et al., 2002 ; Lapierre et al., 2004).
In addition, the transgenic lines have also integrated the hygromycin phosphotransferase (hpt) gene that confers resistance to the antibiotic hygromycin. This antibiotic resistance is needed during in vitro culture steps to select cells that have been genetically modified from those that have not been modified.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-101269-4 Organism Populus tremula x Populus alba (Gray Poplar)Trees
Cultivar/ Breeding line: 717-1B4
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pBIBHygro
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-102122-4 Cinnamoyl coenzymeA reductase | Populus tremula x Populus alba (Gray Poplar)Protein coding sequence | Changes in quality and/or metabolite content (Lignin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100292-5 Hygromycin B phosphotransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to antibiotics (Hygromycin)
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
The poplar ccr gene derives from cDNA sequences isolated from a xylem cDNA library from the Populus trichocarpa "Trichobel" clone. The full-length cDNA coding for CCR - Cinnamoyl Coenzyme A reductase (accession AJ224986 ; Leplé et al., 1998) is inserted in sense orientation between i) the promoter of the cauliflower mosaic virus (CaMV) in a duplicated version (a.k.a. p70) and ii) a terminator sequence, either from the T7 gene from the T-DNA (T7 terminator) or from the gene coding for the CaMV 35S RNA (T-35S).
The insertion of the ccr gene in the sense orientation aims at reducing the activity of the target enzyme, through a mechanism named co-suppression.
The resulting gene construct (p70-S-CCR-pA35S) once introduced in the pBIBHygro binary vector generates the pBIBHygro/SCCR pBIBHygro transformation vector.
The antibiotic resistance gene hygromycine B phosphotransferase (hpt or hph) was fused to the promoter of the nopaline synthase gene (P-nos) and to the terminator of the T7 gene of the Ti plasmid.
The inserted genetic material is the T-DNA from the Ti plasmid of Agrobacterium tumefaciens harbouring the constructs described above.
EN
The insertion of the ccr gene in the sense orientation aims at reducing the activity of the target enzyme, through a mechanism named co-suppression.
The resulting gene construct (p70-S-CCR-pA35S) once introduced in the pBIBHygro binary vector generates the pBIBHygro/SCCR pBIBHygro transformation vector.
The antibiotic resistance gene hygromycine B phosphotransferase (hpt or hph) was fused to the promoter of the nopaline synthase gene (P-nos) and to the terminator of the T7 gene of the Ti plasmid.
The inserted genetic material is the T-DNA from the Ti plasmid of Agrobacterium tumefaciens harbouring the constructs described above.
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- Research
- Biofuel
- Fiber/textile
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