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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Garden Pea modified for the expression of a single chain antibody (scFv) in the seed
EN
BA11
No
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Person:Novoplant GmbHAm Schwabeplan 1BGatersleben,
06466, GermanyPhone: + 49 39482 7998-0,Fax: + 49 39482 7998-80,Email: contact@novoplant.de,Website:Related OrganizationNovoplant GmbH ()Private sector (business and industry)Am Schwabeplan 1BGatersleben,
06466, GermanyPhone: + 49 39482 7998-0,Fax: + 49 39482 7998-80,Email: contact@novoplant.de,Website:
BA11-2 pea plants express seed-specific, within the peas, the antibody fragment scFv BA11. This antibody fragment has affinity towards enterotoxigenic Escherichia coli (ETEC) cells that can cause the postweaning diarrhea by adhesion to the intestinal wall in pigs.
To prevent the outbreak of postweaning diarrhea BA11-expressing peas shell be feed to pigs.
EN
To prevent the outbreak of postweaning diarrhea BA11-expressing peas shell be feed to pigs.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-100289-5 Organism Pisum sativum (Garden pea, PEA)Crops
Pisum sativum var. Eiffel
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pPZP-USP(+)-BA11
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103026-3 Unknown seed protein-promoter | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Promoter
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BCH-GENE-SCBD-103025-2 LeB4 Signal peptide | Vicia faba (Broad Bean, Tick Bean, Windsor Bean, Horse Bean, Pigeon Bean, Field Bean)Transit signal
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BCH-GENE-SCBD-103024-2 Single-chain Fragment variable (scFv) antibody BA11 | Mus musculus (Mouse, House mouse, MOUSE)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Antibodies and antigens)
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BCH-GENE-SCBD-103023-2 KDEL ER retention signalTransit signal
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BCH-GENE-SCBD-103022-3 Hexahistidine tagProtein coding sequence | Protein purification
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
By action of the N-terminal signal peptide LeB4 and the C-terminal KDEL retetion signal the scFv BA11 antibody fragment accumulates within the endoplasmic reticulum but does not enter the secretion pathway. Since the LeB4-scFv BA11-KDEL fusion protein is under the control of the USP(+) promoter seed specific expression is achieved. A N-terminal His-tag allows immuno detection and immunoprecipitation of the fusion protein.
EN
EN
- Pharmaceutical
PCR-based methods, to be developed on information about the transformation vector.
EN
EN
- Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity, [ English ]
- Production of Recombinant Antibodies in Pea Seeds and Their Oral Application in Piglets, Biotechnology and Sustainable Agriculture 2006 and Beyond [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
