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Living Modified Organism
(LMO)
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Barley modified to produce phytase
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SCLW-GP-PHYA
No
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Prague,Person:Dr. Ludmila Ohnoutkovásenior scientist,
, CzechiaPhone: +420 225 106 455,Fax: +420 225 106 456,Email: ueb@ueb.cas.cz,Website: http://www.ueb.cas.cz/en,Related OrganizationInstitute of Experimental Botany AS CR (IEB)Academic or research institutePrague,
, CzechiaPhone: +420 225 106 455,Fax: +420 225 106 456,Email: ueb@ueb.cas.cz,Website: http://www.ueb.cas.cz/en,
SCLW-GP-PHYA barley has been genetically modified by insertion of the fungal phytase gene from Aspergillus niger. Cereals (including barley), oil crops and legumes contain phytic acid, myo-inositolhexakisphosphate that is an antinutritional compound. Ions of iron and cations (manganese, magnesium, zinc and calcium) which are bound to phytic acid are difficult to digest. These complexes negatively affect the usability of phosphorus and bound cations in human and monogastric animal foodstuffs. The enzyme 3-phytase is effectively releasing phosphorus and cations which are bound to phytic acid in barley seeds. The higher-phytase lines show 3.5 fold increase content of pytase activity in barley grain.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12110-5 Organism Hordeum vulgare (Barley, HORVU)Crops
Variety: Golden Promise
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Cotransformation of pAMFIT and pAL70 vectors
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- Biolistic / Particle gun
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15378-5 phyA gene | Aspergillus nigerProtein coding sequence | Changes in quality and/or metabolite content,Phytate degradation
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BCH-GENE-SCBD-104334-2 Alpha-amylase gene promoter | Hordeum vulgare (Barley, HORVU)Promoter
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-104332-2 Firefly Luciferase | Photinus pyralis (North American firefly, Common Eastern firefly, Big Dipper firefly, PHOPY)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
Zygotic immature embryos of spring barley were co-transformed by particle bombardment with combinations of two vectors:
The pAMFIT vector contains the gene phyA from Aspergillus niger.
The pAL70 vector contains the herbicide selection gene (bar) from Streptomyces hygroscopicus, conferring resistance against the plant herbicide Basta, and the common luciferase reporter gene (luc) from Photinus pyralis.
Transgenic plants were regenerated from embryogenic callus selected on Bialaphos containing medium, and analyzed for presence phyA, bar, luc genes by PCR and RT-PCR.
Notes on Genetic Elements:
α-amylase promoter: promoter region from Hordeum vulgare cv. Himalaya gene
bar gene: Under control of ubi promoter, promoter region from Zea mays polyubiquitin gene and first intron (terminator unknown)
luc gene: Under control of ubi promoter, promoter region from Zea mays polyubiquitin gene and first intron (terminator unknown)
EN
The pAMFIT vector contains the gene phyA from Aspergillus niger.
The pAL70 vector contains the herbicide selection gene (bar) from Streptomyces hygroscopicus, conferring resistance against the plant herbicide Basta, and the common luciferase reporter gene (luc) from Photinus pyralis.
Transgenic plants were regenerated from embryogenic callus selected on Bialaphos containing medium, and analyzed for presence phyA, bar, luc genes by PCR and RT-PCR.
Notes on Genetic Elements:
α-amylase promoter: promoter region from Hordeum vulgare cv. Himalaya gene
bar gene: Under control of ubi promoter, promoter region from Zea mays polyubiquitin gene and first intron (terminator unknown)
luc gene: Under control of ubi promoter, promoter region from Zea mays polyubiquitin gene and first intron (terminator unknown)
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- Food
- Feed
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| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Related LMO(s) | 1 | |
