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Living Modified Organism
(LMO)
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Hybrid aspen lines with modified wood development
EN
Multiple transformation events
No
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Helsinki,Person:Division of Plant Biology and Institute of Biotechnology, Department of Biosciences
, FinlandPhone: +358 2941 57773,Fax:Email: jorma.vahala@helsinki.fi,Website:Related OrganizationUniversity of Helsinki ()Academic or research institute
Hybrid aspen were genetically modified to understand the role and function of plant hormones ethylene and cytokinin in wood formation.
In these lines the genetically modified hybrid aspens overexpress Ethylene Response Factor (ERF) genes or a gene enhancing cytokinin signaling under a constitutive LMX5 promoter, which directs the expression to wood forming tissues.
Overexpression of these genes modifies wood properties and enhances the growth.
EN
In these lines the genetically modified hybrid aspens overexpress Ethylene Response Factor (ERF) genes or a gene enhancing cytokinin signaling under a constitutive LMX5 promoter, which directs the expression to wood forming tissues.
Overexpression of these genes modifies wood properties and enhances the growth.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-105731-2 Organism Populus tremula x Populus tremuloides (Hybrid aspen)Trees
Cultivar/breeding line: Clone T89 and clone 51
EN
LMX5
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-105735-2 Ethylene response factor gene | Populus trichocarpa (Western balsam poplar, Black cottonwood, California poplar, POPTR)Protein coding sequence | Changes in physiology and/or production (Yield)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-104314-4 Gene 4 transcription terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100292-5 Hygromycin B phosphotransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to antibiotics (Hygromycin)
LMX5 Vector
Three different ERF genes (ERF21, ERF30 and ERF33) from P. trichocarpa and a gene enhancing cytokinin response from A. thaliana are overexpressed.
The overexpressed genes are regulated by a constitutive LMX5 promoter from hybrid aspen.
The hpt gene conferring hygromycin B resistance. The vector has also ColE1 replicon and β-lactamase-gene for bacterial selection from Escherichia coli pBR322 plasmid that are non-functional in plants.
EN
Three different ERF genes (ERF21, ERF30 and ERF33) from P. trichocarpa and a gene enhancing cytokinin response from A. thaliana are overexpressed.
The overexpressed genes are regulated by a constitutive LMX5 promoter from hybrid aspen.
The hpt gene conferring hygromycin B resistance. The vector has also ColE1 replicon and β-lactamase-gene for bacterial selection from Escherichia coli pBR322 plasmid that are non-functional in plants.
EN
- Timber
EN
EN
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