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Living Modified Organism
(LMO)
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Colour Modified Carnation
EN
30086
Yes
SHD-30086-3
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Person:Dr Yukihisa KatsumotoPrincipal Researcher, Research Institute1-1-1 Wakayamadai, Shimamoto-choMishima-gun, Osaka
618-8503, JapanPhone: +81 75 962 9132,Fax: +81 75 962 3791,Website:Related OrganizationSuntory Holdings Limited (SHD)Private sector (business and industry)1-1-1 Wakayamadai, Shimamoto-choMishima-gun, Osaka
618-8503, JapanPhone: +81 75 962 9132,Fax: +81 75 962 3791,Website:
Carnation variety 30086 flowers have a pastel violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-4954-7 Organism Dianthus caryophyllus (Carnation, DIACA)Crops
EN
pCGP3601
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-103771-1 Chalcone synthase gene promoter | Antirrhinum majus (Common Snapdragon, Snapdragon)Promoter
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BCH-GENE-SCBD-43793-4 Flavonoid 3', 5'-hydroxylase gene | Viola sp. (Pansy, VIOLA)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-103772-2 D8 gene terminator | Petunia hybrida (Petunia, PETHY)Terminator
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BCH-GENE-SCBD-105427-1 Anthocyanidin synthase gene promoter | Dianthus caryophyllus (Carnation, DIACA)Promoter
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BCH-GENE-SCBD-105436-1 Anthocyanin-3',5'-methyltransferase gene | Torenia sp. (Torenia hybrid, Wishbone flowers, Blue Wings, TOREN)Protein coding sequence | Changes in quality and/or metabolite content (Flavonoids (e.g. anthocyanin), Pigmentation / Coloration)
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BCH-GENE-SCBD-105424-1 Anthocyanidin synthase gene terminator | Dianthus caryophyllus (Carnation, DIACA)Terminator
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BCH-GENE-SCBD-104594-4 Dihydroflavonol-4-reductase | Dianthus caryophyllus (Carnation, DIACA)Protein coding sequence | Changes in quality and/or metabolite content (Pigmentation / Coloration)
The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments which are modified by expression of an anthocyanin-3',5'-methyltransferase gene.
A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
An anthocyanin-3',5'-methyltransferase gene has also been added. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce the anthocyanins petunidin-3-glucoside and malvidin-3-glucoside.
EN
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments which are modified by expression of an anthocyanin-3',5'-methyltransferase gene.
A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
An anthocyanin-3',5'-methyltransferase gene has also been added. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce the anthocyanins petunidin-3-glucoside and malvidin-3-glucoside.
EN
- Ornamental
EN
30086 was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing four genes that function together in the biosynthesis of the anthocyanin pigments related to delphinidin.
The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr), a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), and an anthocyanin-3',5'-methyltransferase gene.
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce anthocyanins with a novel hue.
EN
The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr), a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), and an anthocyanin-3',5'-methyltransferase gene.
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce anthocyanins with a novel hue.
