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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Arctic™ "Granny Smith" Apple
EN
GD784
Yes
OKA-NBØØ2-9
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Person:Neal CarterPO Box 1533Summerland, BC
V0H 1Z0, CanadaPhone:Fax:Email:Website:Related OrganizationOkanagan Specialty Fruits Inc. ()
Arctic Apple are modified to resist to enzymatic browning by inserting a chimeric polyphenol oxidase (PPO) suppression element derived from apple PPO sequences. The flesh of modified apples subjected to mechanical damage, such as slicing or bruising, does not brown as compared to a conventional apple.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12099-5 Organism Malus domestica (Apple, MALDO)Crops
Granny Smith
EN
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OKA-NBØØ1-8 - Arctic™ "Golden Delicious" Apple| Okanagan Specialty Fruits Inc. | Changes in quality and/or metabolite content (Pigmentation / Coloration), Resistance to antibiotics (Kanamycin, Neomycin)
GEN-03
EN
- Agrobacterium-mediated DNA transfer
0.650 kb
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0.000 kb
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1.810 kb
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0.260 kb
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0.310 kb
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0.980 kb
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0.260 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103886-2 5' Untranslated Leader of AMV RNA4 | Alfalfa mosaic virus (Alfalfa mosaic virus, AMV)Leader
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BCH-GENE-SCBD-106414-3 PPO Suppression Transgene | Malus domestica (Apple, MALDO)Double-stranded RNA
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
PCR and southern blot analysis indicate that the presence of both the 3' end of the PPO suppression transgene and the NptII selection marker as well as the presence of an intact copy of the PPO suppression portion of the insert.
Southern data, combined with Mendelian Inheritance data indicates that GD784 contains four unlinked insertions. Southern blots also indicates that there is no evidence for inclusion of a complete copy of the vector backbone in the modified genome.
EN
Southern data, combined with Mendelian Inheritance data indicates that GD784 contains four unlinked insertions. Southern blots also indicates that there is no evidence for inclusion of a complete copy of the vector backbone in the modified genome.
EN
- Food
EN
EN
- OKA-NBØØ2-9 - APHIS [ English ]
- OKA-NBØØ2-9 - ISAAA [ English ]
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