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Living Modified Organism
(LMO)
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Canola modified for herbicide tolerance
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aroA, thiO
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Person:Анатолий Николаевич ЕвтушенковHead of the Department of Molecular Biology, Department of Molecular BiologyKurchatova, 10Minsk,
, BelarusPhone: +375 17 2095898,Fax:Email: evtushenkov@bsu.by,Website:Related OrganizationFaculty of Biology of the Belarusian State University ()Academic or research institute
Canola was modified with the insertion of the aroA gene from Dickeya dadantii encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and the thiO gene from Bacillus subtilis which encodes a glycine oxidase. Te transformation of these genes into the canola genome confers resistance to the herbicide glyphosate.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12083-7 Organism Brassica napus (Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA)Crops
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Canola modified for herbicide tolerance| Faculty of Biology of the Belarusian State University | Resistance to antibiotics (Kanamycin), Resistance to herbicides (Glyphosate)
pZH513 constructed from the pGreen vector by inserting the Dickeya dadantii aroA gene and the Bacillus subtilis thiO gene into the T-region
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-106246-1 5-enolpyruvylshikimate-3-phosphate synthase gene | Dickeya dadantii (DICDA)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-108046-4 Glycine Oxidase gene | Bacillus subtilis (Bacillus, BACIU)Protein coding sequence | Resistance to herbicides (Glyphosate)
Incorporated sequences were modified: two mutations in the gene aroA (P101S, T971) and three mutations in the gene thiO (G51S, A54R, H244R).
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- Feed
- Biofuel
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