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Living Modified Organism
(LMO)
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Potato modified for pathogen resistance
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Solanum tuberosum L, variety Scarb N
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Person:Анатолий Николаевич ЕвтушенковHead of the Department of Molecular Biology, Department of Molecular BiologyKurchatova, 10Minsk,
, BelarusPhone: +375 17 2095898,Fax:Email: evtushenkov@bsu.by,Website:Related OrganizationFaculty of Biology of the Belarusian State University ()Academic or research institute
Intracellular expression of the transgenic hrpN gene has been shown to upregulate the expression of endogenous PR2, PR5 and PR10 genes which may confer increased protection against biotrophic pathogens in transgenic Solanum tuberosum L. plants as compared to control.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Variety: Scarb N
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pBIP-N constructed on the basis of pBI121 binary vector by insertion of hrpN-FLAG sequence into T-region
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- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-108047-2 Harpin protein coding sequence | Pectobacterium carotovorum (PECCA)Protein coding sequence | Resistance to diseases and pests
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
The hrpN sequence is modified at the 3 'end by addition of a short (8 codons) FLAG-epitope, allowing easy detection of its product by means of commercial anti-FLAG antibodies.
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- Research
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- Analysis of hrpN expression in tomato plants (in Russian) [ English ]
- АНАЛИЗ ДЕЙСТВИЯ ХАРПИНОВ НА РАСТЕНИЯ ТОМАТА [ Russian ]
- Expression of bacterial gene hrpNPc in potato plants changes expression pattern of the immune system`s genes (in Russian) [ English ]
- ЭКСПРЕССИЯ БАКТЕРИАЛЬНОГО ГЕНА HRPNPC В РАСТЕНИЯХ КАРТОФЕЛЯ ИЗМЕНЯЕТ ЭКСПРЕССИОННЫЙ ФОН ГЕНОВ ИММУННОЙ СИСТЕМЫ О.К. Присяжненко [ Russian ]
- Expression of HarpinXoo in Transgenic Tobacco Induces Pathogen Defense in the Absence of Hypersensitive Cell Death [ English ]
- Harpin-elicited hypersensitive cell death and pathogen resistance require the NDR1 and EDS1 genes [ English ]
- Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. [ English ]
