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Living Modified Organism (LMO)
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BCH-LMO-SCBD-109049-3   |   PDF   |   Print   |  
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Decisions on the LMO Risk Assessments  
published: 16 Oct 2015 last updated: 15 Aug 2024
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Eucalyptus modified for increased growth and yield
EN
H421
Yes
FGN-Ø1A21-5
Hybrid eucalyptus (Eucalyptus grandis × Eucalyptus urophylla) was modified for greater biomass yield through the overexpression of Arabidopsis thaliana endo-β‐1,4‐d-glucanase, which remodels the xyloglucan-cellulose matrix of the cell wall during development to promote cell expansion and growth. Thus, the transgene increases the rate of wood growth and yield of the tree. In addition, the modified eucalyptus contains an Escherichia coli neomycin phosphotransferase II cassette, which functioned as a selectable marker during transformation of the plants.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pBI121
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-109045-1 Strawberry vein banding virus promoter | Strawberry vein banding virus (SVBV)
    Promoter
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-109046-1 Endo‐1,4‐β‐d‐glucanase coding sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Protein coding sequence | Changes in physiology and/or production (Yield)
The modified eucalyptus contains two gene cassettes: Arabidopsis thaliana endo-β‐1,4‐d-glucanase (cel1) and Escherichia coli neomycin phosphotransferase II (nptII).

The cel1 sequence is under control of a Strawberry vein banding virus promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the viral promoter, which induces strong constitutive transcription.

The nptII sequence is under control of a nopaline synthase promoter and terminator. 

Note:
  • Molecular analysis (Southern blot, sequencing and PCR) showed that a single T-DNA insertion occurred into the eucalyptus genome. 
  • Sequencing of event H421 confirmed that constructs present in the vector were preserved in the transformation process (without rearrangement of the gene cassettes or truncation of the inserted T-DNA sequence).
EN
LMO characteristics
EN
  • Timber
Detection method(s)
EN
Records referencing this document Show in search
Record type Field Record(s)
Living Modified Organism Recipient Organism or Parental Organisms 3
Country's Decision or any other Communication LMO identification 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1

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