Sugar beet modified for herbicide tolerance | BCH-LMO-SCBD-109070 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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BCH-LMO-SCBD-109070-2   |   PDF   |   Print   |  
Decisions on the LMO Risk Assessments  
published: 21 Oct 2015 last updated: 26 Mar 2020
Living Modified Organism identity
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Sugar beet modified for herbicide tolerance
EN
T227-1
No
The T227-1 sugar beet (Beta vulgaris) was modified with the insertion of Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) to confer herbicide tolerance to the glyphosate. The variation in the bacterial enzyme allows the plant to tolerate otherwise lethal doses of glyphosate.

Note:
The beet lines T227-1 and H7 (Record ID: 14773 - see below) are independent transformation lines containing the same genetic elements.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
EN
Characteristics of the modification process
pMON17227
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)
    Promoter
  • BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)
    Transit signal
  • BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)
    Protein coding sequence | Resistance to herbicides (Glyphosate)
  • BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)
    Terminator
Transcription of the Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene is under control of the Figwort Mosaic Virus 35S promoter and the Pisum sativum Rubisco small subunit (rbcS-E9) terminator. The transcript contains an Arabidopsis thaliana chloroplast transit peptide at the 5' end of the transcript to facilitate post-translational targeting to the chloroplast. Due to the viral promoter, transcription is expected to occur constitutively at elevated levels.

Please note: the nucleic acid sequence of the epsps gene was codon-optimized for expression in plants.
EN
LMO characteristics
EN
  • Research
Detection method(s)
EN
Additional Information
The sugar beet lines T227-1 and H7-1 are independent transformation events harbouring the same genetic elements. 
EN
Records referencing this document Show in search
Record type Field Record(s)
Country's Decision or any other Communication Living modified organism(s) 1
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Living Modified Organism Related LMO(s) 1