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Genetic element
(GENE)
last updated: 10 Nov 2015
Chimeric wheat gliadin
EN
IR-Gli-SYNTH
EN
Double-stranded RNA
Yes
Two fragments of 170 and 191 bp, corresponding to the most conserved regions from, respectively, α- and ω-gliadins, were selected for use as interfering RNA (IR) fragments. These fragments were amplified from bread wheat, assembled to obtain the full chimeric 361-bp ω/α fragment, cloned into the recombination sites of pCR8/GW/TOPO vector (Invitrogen), and recombined to provide the final pDhp-ω/α vector.
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Living Modified Organism | Introduced or modified genetic element(s) | 1 | |
| Living Modified Organism | Genetic elements construct | 1 | |
