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Living Modified Organism
(LMO)
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Potato modified for increased yield and tuber starch content.
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BG1BA-24, BG1BA-31 and BG1BA-32
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Person:Uni Köln, Botanisches InstitutAlbert-Magnus-PlatzKöln, Nordrhein-Westfalen
50923, GermanyPhone: + 49 221 470-2473,Fax: + 49 221 470-5062,Website: http://www.botanik.uni-koeln.de/,Related OrganizationUniversität zu Köln ()Academic or research instituteAlbert-Magnus-PlatzKöln, Nordrhein-Westfalen
50923, GermanyPhone: + 49 221 470-2473,Fax: + 49 221 470-5062,Website: http://www.botanik.uni-koeln.de/,
In the modified potato lines BG1BA-24, BG1BA-31 and BG1BA-32 were engineered to tissue-specifically express the gpt-gene, from P. sativum, and the ntt-gene, from [i]A. thaliana,[/] in the potato tubers, with the possibility of sucrose induced expression in the shoot region.
The gpt gene from pea (Pisum sativum) codes for a plastid glucose-6-phosphate/phosphate translocator, an antiporter whose main physiological function is to import glucose-6-phosphate, in exchange for anorganic phosphate, into the plastids of heterotrophic tissue. Here, it is responsible for the energy-dependent synthesis of starch, using glucose-6-phosphate as a precursor.
The ntt gene from Arabidopsis thaliana codes for a plastid nucleotide translocator. This is also an antiporter which catalyses the plastid uptake of ATP in exchange for ADP and is consequently also referred to as the ATP/ADP translocator. Its main function is to supply non-green storage plastids with ATP, which is needed there for starch synthesis.
As a result of the genetic modification, an increase in the transport activities of glucose-6-phosphate and ATP is expected, which in turn results in an increased tuber yield as well as an increase in the starch content in the tubers of the genetically modified potato plants.
EN
The gpt gene from pea (Pisum sativum) codes for a plastid glucose-6-phosphate/phosphate translocator, an antiporter whose main physiological function is to import glucose-6-phosphate, in exchange for anorganic phosphate, into the plastids of heterotrophic tissue. Here, it is responsible for the energy-dependent synthesis of starch, using glucose-6-phosphate as a precursor.
The ntt gene from Arabidopsis thaliana codes for a plastid nucleotide translocator. This is also an antiporter which catalyses the plastid uptake of ATP in exchange for ADP and is consequently also referred to as the ATP/ADP translocator. Its main function is to supply non-green storage plastids with ATP, which is needed there for starch synthesis.
As a result of the genetic modification, an increase in the transport activities of glucose-6-phosphate and ATP is expected, which in turn results in an increased tuber yield as well as an increase in the starch content in the tubers of the genetically modified potato plants.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar/breeding line: Désirée
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pBinB33(Hyg)::GPT: and pBinB33(Kan)::NTT:
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-110671-1 Glucose-6-phosphate/phosphate-translocator gene | Pisum sativum (Garden pea, PEA)Protein coding sequence | Changes in physiology and/or production (Yield),Changes in quality and/or metabolite content (Carbohydrates)
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BCH-GENE-SCBD-110674-1 Plastidic nucleotide transporter gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Changes in physiology and/or production (Yield),Changes in quality and/or metabolite content (Carbohydrates)
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100292-5 Hygromycin B phosphotransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to antibiotics (Hygromycin)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
The potato plants described here are transformed with two different constructs:
pBinB33(Hyg)::GPT:
The plasmid pBinB33(Hyg)::GPT: used for transformation includes a GPT-expression construct consisting of the B33-promotor of the patatin class I gene from Solanum tuberosum, the glucose-6-phosphate/phosphate-translokator (gpt)-gene from Pisum sativum and the ocs-terminator from Agrobacterium tumefaciens. The hygromycin phosphotransferase gene (hpt) of Streptomyces hygroscopicus expressed under the control of the nos-promotor of the pTiT37 plasmid from A. tumefaciens and the terminator of the gene 7 from A. tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
pBinB33(Kan)::NTT:
The plasmid pBinB33(Kan)::NTT: used for transformation includes a NTT-expression construct consisting of the B33-promotor of the patatin class I gene from S. tuberosum, the nukleotide-translokator (ntt)-gene from Arabidopsis thaliana and the ocs-terminator from A. tumefaciens. A part of the ornithin-cyclo-deaminase (ocd)-gene from A. tumefaciens in front of the neomycin phosphotransferase II gene (nptII) of the Tn5-Transposon of E. coli expressed under the control of the nos-promotor of the pTiT37 plasmid from A. tumefaciens and the terminator of the gene 7 from A. tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
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pBinB33(Hyg)::GPT:
The plasmid pBinB33(Hyg)::GPT: used for transformation includes a GPT-expression construct consisting of the B33-promotor of the patatin class I gene from Solanum tuberosum, the glucose-6-phosphate/phosphate-translokator (gpt)-gene from Pisum sativum and the ocs-terminator from Agrobacterium tumefaciens. The hygromycin phosphotransferase gene (hpt) of Streptomyces hygroscopicus expressed under the control of the nos-promotor of the pTiT37 plasmid from A. tumefaciens and the terminator of the gene 7 from A. tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
pBinB33(Kan)::NTT:
The plasmid pBinB33(Kan)::NTT: used for transformation includes a NTT-expression construct consisting of the B33-promotor of the patatin class I gene from S. tuberosum, the nukleotide-translokator (ntt)-gene from Arabidopsis thaliana and the ocs-terminator from A. tumefaciens. A part of the ornithin-cyclo-deaminase (ocd)-gene from A. tumefaciens in front of the neomycin phosphotransferase II gene (nptII) of the Tn5-Transposon of E. coli expressed under the control of the nos-promotor of the pTiT37 plasmid from A. tumefaciens and the terminator of the gene 7 from A. tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
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- Research
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EN
- B/DE/05/175 - SNIF [ English ]
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