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Living Modified Organism
(LMO)
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Sinorhizobium meliloti modified for the expression of a reporter gene
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L33
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Person:Uni BielefeldUniversitätsstraße 25 33615 BielefeldBielefeld,
33615, GermanyPhone: +49 521 106-00,Fax: +49 521 106-5844,Email: post@uni-bielefeld.de,Related OrganizationUniversität Bielefeld ()Academic or research instituteUniversitätsstraße 25 33615 BielefeldBielefeld,
33615, GermanyPhone: +49 521 106-00,Fax: +49 521 106-5844,Email: post@uni-bielefeld.de,
The genetically modified sinorhizobia constitutively express the luciferase gene from Photinus pyralis coding for the luciferin-4-monooxygenase which catalyses a light-producing reaction and is therefore used as a reporter gene. Other than the expression of the reporter gene, the modified S. meliloti does not differ from the original strain.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-111563-1 Organism Sinorhizobium meliloti (RHIML)Bacteria
The S. meliloti strain Rm2011 was used for the transformation.
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Sinorhizobium meliloti modified for the expression of a reporter gene and reduced growth| Universität Bielefeld | Changes in physiology and/or production (Growth rate), Selectable marker genes and reporter genes
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- Direct DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-104332-2 Firefly Luciferase | Photinus pyralis (North American firefly, Common Eastern firefly, Big Dipper firefly, PHOPY)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-111595-1 Neomycin phosphotransferase II promoter | Escherichia coli (ECOLX)Promoter
The construct did not contain its own termination sequence. It was site-specifically integrated downstream of the S. meliloti recA Gene. The insertion site is located just before the endogenous recA termination region, therefore, transcription termination would be controlled by that sequence.
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- Research
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S. meliloti needs a host plant to grow and proliferate properly. Here, the symbiotic interaction partner alfalfa, Medicago sativa, is used as a host plant.
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- Reduced survival of a RecA-deficient Sinorhizobium meliloti strain in sterile and non-sterile soil during heat stress. [ English ]
- Growth and nodulation competitiveness of Sinorhizobium meliloti L1 (RecA−) is less than that of its isogenic strain L33 (RecA+) but comparable to that of two S. meliloti wild-type isolates. [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
| Living Modified Organism | Related LMO(s) | 1 | |
