Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready Flex® Pima cotton
EN
MON88913
No
-
Person:Regulatory AffairsPO Box 6051St Kilda Road Central, VIC
8008, AustraliaPhone: +61 3 9522 7122,Fax:Email: melbourne.reception@monsanto.com,Website: http://www.monsanto.com/global/au/,Related OrganizationMonsanto Australia ()Private sector (business and industry)PO Box 6051St Kilda Road Central, VIC
8008, AustraliaPhone: +61 3 9522 7122,Fax:Email: melbourne.reception@monsanto.com,Website: http://www.monsanto.com/global/au/,
Roundup Ready Flex® Pima cotton was produced via conventional breeding to transfer the genetic modification from Roundup Ready Flex® cotton (Gossypium hirsutum; MON88913) to unmodified G. barbadense, also known as pima cotton.
Roundup Ready Flex® Pima cotton therefore contains the same modification as MON88913, namely a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the cotton to survive otherwise lethal applications of glyphosate. The cp4 epsps gene introduced into MON 88913 and transferred by conventional breeding to Roundup Ready Flex® Pima cotton was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the CP4 EPSPS enzyme expressed by this gene is tolerant to glyphosate.
As with the MON88913 parent, Roundup Ready Flex® Pima cotton contains two copies of the cp4 epsps gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.
EN
Roundup Ready Flex® Pima cotton therefore contains the same modification as MON88913, namely a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the cotton to survive otherwise lethal applications of glyphosate. The cp4 epsps gene introduced into MON 88913 and transferred by conventional breeding to Roundup Ready Flex® Pima cotton was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the CP4 EPSPS enzyme expressed by this gene is tolerant to glyphosate.
As with the MON88913 parent, Roundup Ready Flex® Pima cotton contains two copies of the cp4 epsps gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-LMO-SCBD-15168-17 Living Modified Organism MON-88913-8 - Roundup Ready™ Flex™ cottonMonsanto | Resistance to herbicides (Glyphosate)
-
BCH-ORGA-SCBD-104688-4 Organism Gossypium barbadense (Sea-island cotton, Egyptian cotton, GOSBA)Crops
G. barbadense cultivar Sipima 280 in Australia; G. barbadense cultivar W2490 in the United States
EN
PV-GHGT35
EN
- Cross breeding
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
|
0.000 kb
|
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
-
BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
-
BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Leader
-
BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
-
BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
-
BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
-
BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)Leader
-
BCH-GENE-SCBD-103907-3 Actin 8 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
-
BCH-GENE-SCBD-103908-4 Actin 8 Leader sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Leader sequence
-
BCH-GENE-SCBD-103909-3 Actin 8 Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
-
BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Leader
DNA insert from MON88913 vector PV-GHGT35
Monsanto constructed the double border, binary plasmid vector PV-GHGT35 for the transformation of G. hirsutum cotton. The resulting modification was then transferred to G. barbadense cotton by conventional breeding.
The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two codon optimised cp4epsps gene cassettes: 1) the first cp4 epsps coding sequence (CS-cp4 epsps) under the regulation of a chimeric transcriptional promoter (P-FMV/Tsf1),Tsf1 leader and intron sequences (L-Tsf1 and I-Tsf1, respectively), a chloroplast transit peptide sequence (TS-ctp2) and a E9 transcript termination and polyadenylation sequence (T-E9) and 2) the second cp4 epsps coding sequence regulated by a chimeric transcriptional promoter(P-35S/act8), act8 leader and intron sequences (L-act8, and I-act8,respectively) and the same chloroplast targeting and transcript termination sequences as utilized in the first cp4 epsps gene expression cassette.
For additional information on this LMO, please refer to the records of the parental LMO.
EN
Monsanto constructed the double border, binary plasmid vector PV-GHGT35 for the transformation of G. hirsutum cotton. The resulting modification was then transferred to G. barbadense cotton by conventional breeding.
The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two codon optimised cp4epsps gene cassettes: 1) the first cp4 epsps coding sequence (CS-cp4 epsps) under the regulation of a chimeric transcriptional promoter (P-FMV/Tsf1),Tsf1 leader and intron sequences (L-Tsf1 and I-Tsf1, respectively), a chloroplast transit peptide sequence (TS-ctp2) and a E9 transcript termination and polyadenylation sequence (T-E9) and 2) the second cp4 epsps coding sequence regulated by a chimeric transcriptional promoter(P-35S/act8), act8 leader and intron sequences (L-act8, and I-act8,respectively) and the same chloroplast targeting and transcript termination sequences as utilized in the first cp4 epsps gene expression cassette.
For additional information on this LMO, please refer to the records of the parental LMO.
EN
- Fiber/textile
EN
The EPSPS enzyme is part of the shikimate pathway, an important biochemical pathway in plants involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed for growth and survival. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, since these organisms are unable to synthesize their own aromatic amino acids. Because the aromatic amino acid pathway is not present in mammals, birds, or aquatic life forms, glyphosate has little, if any, toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
EN
| Record type | Field | Record(s) | |
|---|---|---|---|
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
| Country's Decision or any other Communication | LMO identification | 1 | |
