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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Pinkglow™ pineapple
EN
EF2-114
Yes
FDP-ØØ114-5
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Organization:Del Monte Fresh Produce Company ()Private sector (business and industry)241 Sevilla AvenueCoral Gables, Florida
33134, United States of AmericaPhone: +1 305-520-8400, +1 800-950-3683,Fax: +1 305-567-0320,Website: https://freshdelmonte.com/,
The pineapple (Ananas comosus) was modified to alter the color of the fruit from yellow to pink through accumulation of lycopene in place of β-carotene, and to introduce control of flowering. The modification of fruit colour was carried out through the insertion of a gene coding for Citrus unshiu phytoene synthase, which is involved in a key step in lycopene synthesis, and two RNAi constructs that were designed to suppress endogenous expression of each of lycopene β-cyclase and lycopene ε-cyclase, which convert lycopene to other carotenoids. To control flowering of the pineapple, an RNAi construct was inserted, which is intended to suppress endogenous expression of 1-aminocyclopropane-1-carboxylic acid synthase, the penultimate step in ethylene biosynthesis (which induces flowering in pineapples). Additionally, Nicotania tabacum acetohydroxyacid synthase cassettes were also added to act as a marker for sulfonylurea (chlorsulfuron) selection during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-111928-1 Organism Ananas comosus (Pineapple, ANACO)Crops
Variety: MD2
EN
pHCW.T-7 and pHCWflACC3ʹ-2
EN
- Agrobacterium-mediated DNA transfer
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1.700 kb
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1.700 kb
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2.557 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-111929-2 Epoxide hydrolase promoter | Ananas comosus (Pineapple, ANACO)Promoter
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BCH-GENE-SCBD-111930-1 Tetrameric ubiquitin gene promoter | Ananas comosus (Pineapple, ANACO)Promoter
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
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BCH-GENE-SCBD-111931-2 Bromelain inhibitor gene promoter | Ananas comosus (Pineapple, ANACO)Promoter
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BCH-GENE-SCBD-111933-1 Phytoene synthase gene | Citrus unshiu (Satsuma mandarin, Satsuma orange, Tangerine, CITUN)Protein coding sequence | Changes in quality and/or metabolite content (Vitamins)
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BCH-GENE-SCBD-111934-1 Tetrameric ubiquitin gene terminator | Ananas comosus (Pineapple, ANACO)Terminator
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BCH-GENE-SCBD-111935-2 Lycopene beta cyclase fragment | Ananas comosus (Pineapple, ANACO)Double-stranded RNA | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-111936-2 Light-inducible tissue-specific LS1 intron 2 | Solanum tuberosum (Potato, SOLTU)Intron
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BCH-GENE-SCBD-111937-2 Lycopene epsilon-cyclase fragment | Ananas comosus (Pineapple, ANACO)Double-stranded RNA | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-111938-2 1-aminocyclopropane-1-carboxylic acid synthase fragment | Ananas comosus (Pineapple, ANACO)Double-stranded RNA
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-111929-2 Epoxide hydrolase promoter | Ananas comosus (Pineapple, ANACO)Promoter
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BCH-GENE-SCBD-111930-1 Tetrameric ubiquitin gene promoter | Ananas comosus (Pineapple, ANACO)Promoter
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BCH-GENE-SCBD-15177-7 Acetohydroxy acid synthase gene | Nicotiana tabacum (Tobacco, TOBAC )Protein coding sequence | Resistance to herbicides (Chlorsulfuron, Sulfonylurea)
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BCH-GENE-SCBD-100390-7 Acetohydroxy acid synthase gene terminator | Nicotiana tabacum (Tobacco, TOBAC )Terminator
DNA insert from vector pHCW.T-7
Vector pHCW.T-7 contains four expression cassettes:
1) The Nicotania tabacum acetohydroxyacid synthase (SuRB) expression cassette, intended to function as a plant selectable marker, which is composed of:
Vector pHCW.T-7 contains four expression cassettes:
1) The Nicotania tabacum acetohydroxyacid synthase (SuRB) expression cassette, intended to function as a plant selectable marker, which is composed of:
- constitutive fusion promoter, comprising the promoter/5ʹ untranslated region (UTR) of the epoxide hydrolase (ehs) gene and the promoter/5ʹ UTR of the tetrameric ubiquitin (ubi) gene, both isolated from pineapple (Ananas comosus);
- SuRB a mutant acetolactate synthase (als) gene from tobacco (Nicotiana tabacum); and
- A transcription terminator sequence corresponding to the 3ʹ untranslated region of the tobacco als.
2) The BRIp-PSY-Ubpter expression cassette intended to express the phytoene synthase protein, which is composed of:
- A modified promoter derived from the pineapple bromelain (bi) gene;
- The tangerine (Citrus unshiu) phytoene synthase (psy) gene; and
- The transcription terminator sequence corresponding to the 3ʹ UTR and flanking region of the pineapple ubi.
3) The lycopene β-cyclase (lycb) RNA interference (RNAi) cassette, intended to suppress expression of the pineapple lycopene β-cyclase protein, which is composed of:
- A modified pineapple ubi promoter;
- A partial coding sequence of pineapple lycb in the sense orientation;
- The intron 2 sequence of the potato (Solanum tuberosum) light-inducible tissue-specific LS1 (psbr) gene;
- A partial coding sequence of pineapple lycb in the anti-sense orientation; and
- The transcription terminator sequence corresponding to the 3ʹ UTR and flanking region of the pineapple ubi.
4) The lycopene ε-cyclase (lyce) RNAi cassette, intended to suppress expression of the pineapple lycopene ε-cyclase protein, which is composed of:
- A modified promoter derived from the pineapple bi;
- A partial coding sequence of pineapple lyce in the sense orientation;
- The intron 2 sequence of the potato psbr;
- A partial coding sequence of pineapple lyce in the anti-sense orientation; and
- The transcription terminator sequence corresponding to the 3ʹ UTR and flanking region of the pineapple ubi.
DNA insert from vector pHCWflACC3ʹ-2
Vector pHCWflACC3ʹ-2 contains two expression cassettes:
1) The SuRB expression cassette described above, intended as a plant selectable marker
2) The 1-aminocyclopropane-1-carboxylic acid synthase (acs) RNAi cassette, intended to suppress expression of acs, which is composed of:
Vector pHCWflACC3ʹ-2 contains two expression cassettes:
1) The SuRB expression cassette described above, intended as a plant selectable marker
2) The 1-aminocyclopropane-1-carboxylic acid synthase (acs) RNAi cassette, intended to suppress expression of acs, which is composed of:
- A promoter sequence corresponding to the promoter/5ʹ region of the pineapple ubi;
- A partial coding sequence of pineapple meristem acs in the sense orientation;
- The intron 2 sequence of the potato psbr;
- A partial coding sequence of pineapple meristem acs in the anti-sense orientation; and
- The transcription terminator sequence corresponding to the 3ʹ UTR and flanking region of the pineapple ubi.
Characteristics of the introduced DNA
Southern blot analysis was carried out to determine the characteristics and stability of the introduced DNA. Studies provided data that was consistent with four complete integrations of the pHCW.T-7 T-DNA, one irregular integration of the pHCWflACC3ʹ-2 T-DNA, and three partial integrations of the pHCWflACC3ʹ-2 right border region.
Furthermore, the data indicated that at least one intact copy of pHCW.T-7 is present in the EF2-114 genome, in addition to rearranged copies of pHCW.T-7, the exact structure of which could not be completely elucidated. In addition the data supports that no intact copies of pHCWflACC3ʹ-2 are present in EF2-114, and that extensive rearrangements of pHCWflACC3ʹ-2 genetic elements had occurred.
Copy number analysis was consistent with the integration of four copies of the pHCW.T-7 plasmid, of which two were complete. There was also evidence of one partial integration of the pHCWflACC3ʹ-2 plasmid.
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BCH-GENE-SCBD-111935-2 Lycopene beta cyclase fragment | Ananas comosus (Pineapple, ANACO)Double-stranded RNA | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-111937-2 Lycopene epsilon-cyclase fragment | Ananas comosus (Pineapple, ANACO)Double-stranded RNA | Changes in quality and/or metabolite content (Pigmentation / Coloration)
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BCH-GENE-SCBD-111938-2 1-aminocyclopropane-1-carboxylic acid synthase fragment | Ananas comosus (Pineapple, ANACO)Double-stranded RNA
The modified pineapple contains three RNA interference (RNAi) cassettes intended to silence the expression of the endogenous lycopene beta-cyclase, lycopene epsilon-cyclase and 1-aminocyclopropane-1-carboxylic acid genes. The RNAi cassettes contain sense and anti-sense separated by an intron. After transcription, the sense and anti-sense sequences will base pair due to complementarity and form a hairpin (hairpin RNA; hpRNA). The double stranded structure is then recognized by host DICER-like endonucleases, which process the hpRNA into small interfering RNA (siRNA) roughly 21 - 23 nucleotides in length. Argonaute proteins then recruit the siRNA to target mRNA molecules with sequence complementarity to the siRNA for degradation or translational repression, resulting in silencing of the specific genes.
- Food
EN
The modified pineapple is also known by the name "Rosé".
- FDA - Pineapple with altered color containing lycopene and reduced ripening [ English ]
- Pineapple plant named ‘Rosé’ - Patent [ English ]
- EUginius - EF2-114 pineapple [ English ]
- DelMonte - Pinkglow Pineapple [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 1 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 1 | |
