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Living Modified Organism (LMO)
  |  
Decisions on the LMO Risk Assessments  
last updated: 05 Oct 2017
Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Cassava modified for reduced starch metabolism
EN
AMY3 RNAi
No
Cassava was genetically modified for reduced starch metabolism in the tuber after pruning, prior to crop harvest.

The modification was carried out through the insertion of an RNAi construct that was designed to suppress endogenous expression of each of alpha-amylase 3 which is involved in is involved in the release of branched malto‐olicosaccharides from the starch granule.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Cultivar 60444
EN
Characteristics of the modification process
pCAMBIA1301_patatin AMY3 RNAi
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
  • BCH-GENE-SCBD-100273-4 B33 gene promotor | Solanum tuberosum (Potato, SOLTU)
    Promoter
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
  • BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)
    Terminator
  • BCH-GENE-SCBD-112601-1 M27939 Intron Sequence
    Intron
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-112606-1 Alpha-amylase 3 gene | Manihot esculenta (Cassava, Brazilian arrowroot, Yuca, Manioc, Mandioca, MANES)
    Protein coding sequence | Changes in quality and/or metabolite content (Carbohydrates)
Base pairs 57‐165 of the synthetic plant intron sequence M27939 are integrated into the genetic construct.

The α-amylase sequence consist of the first 210 base pairs of the coding sequence.
EN
LMO characteristics
EN
  • Food
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Country's Decision or any other Communication LMO identification 1