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Genetic element
(GENE)
published: 10 May 2019
last updated: 19 Jun 2019
Recombinant glycolate dehydrogenase (fused subunits DEF)
EN
-
DEFpEN
GlyDH
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-14930-5 Organism Escherichia coli (ECOLX)Bacteria
EN
EN
Glycolate dehydrogenase converts 2-phosphoglycolate to glycerate and CO2. In plant biotechnology applications, the enzyme can be used used to increase photosynthetic rates by reducing the the toxic effects of photorespiration (Ribulose-1,5-bisphosphate-caboxylase/-oxygenase (Rubisco) to binding O2 instead of CO2).
The recombinant glycolate dehydrogenase polyprotein (DEFp) by fusing the corresponding E. coli glcD (1493bps),glcE (1046 bps), and glcF (1220 bps) cDNAs with intervening flexible (Gly4Ser) 3 linkers. The fused polyprotein ensures the protein subunits are produced in stoichiometric amounts.
EN
The recombinant glycolate dehydrogenase polyprotein (DEFp) by fusing the corresponding E. coli glcD (1493bps),glcE (1046 bps), and glcF (1220 bps) cDNAs with intervening flexible (Gly4Ser) 3 linkers. The fused polyprotein ensures the protein subunits are produced in stoichiometric amounts.
EN
- A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa.pdf [ English ]
- The expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids strongly enhances photosynthesis and tuber yield [ English ]
- KEGG Pathway - Glycolate dehydrogenase [ English ]
- UniProt - E. coli glcD [ English ]
- UniProt - E. coli glcE [ English ]
- UniProt - E. coli glcF [ English ]
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