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Living Modified Organism
(LMO)
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Maize with impaired DNA-repair mechanism
EN
B104 (ATR-KO)
No
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Person:Dr. Hilde Nelissen, Departement PlantensysteembiologieTechnologiepark 927Gent,
B-9052, BelgiumPhone: +32 9 2446611,Fax:Email: vib@vib.be,Website: http://www.vib.be,Related OrganizationVIB - Universiteit Gent (VIB-UGent)Academic or research instituteTechnologiepark 927Gent,
B-9052, BelgiumPhone: +32 9 2446611,Fax:Email: vib@vib.be,Website: http://www.vib.be,
The maize plants have been edited using the CRISPR/Cas9 system resulting in the mutation of the ATR gene. One extra DNA base pair has been introduced into the target gene resulting in the knock-out of this gene by means of a frameshift mutation.
The ATR gene plays a role in the repair of DNA damage. Thus, DNA damage is expected to accumulate to a higher degree due to the faulty repair mechanism. The plants could act as a biosensor for DNA damage caused by different forms of abiotic stress such as drought, heat, or environmental pollution.
EN
The ATR gene plays a role in the repair of DNA damage. Thus, DNA damage is expected to accumulate to a higher degree due to the faulty repair mechanism. The plants could act as a biosensor for DNA damage caused by different forms of abiotic stress such as drought, heat, or environmental pollution.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
Zea Mays inbred line B104
EN
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Maize with impaired DNA-repair mechanism| Impaired DNA repair, Sensitivity to DNA damaging agents, Tolerance to abiotic stress (Cold / Heat, Drought)
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Maize with impaired DNA-repair mechanism| Impaired DNA repair, Sensitivity to DNA damaging agents, Tolerance to abiotic stress (Cold / Heat, Drought)
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Maize with impaired DNA-repair mechanism| Impaired DNA repair, Sensitivity to DNA damaging agents, Tolerance to abiotic stress (Cold / Heat, Drought)
pBUN411
EN
- Agrobacterium-mediated DNA transfer
- Other (CRISPR-Cas9 technique)
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-115116-2 ATR serine/threonine kinase | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Impaired DNA repair,Sensitivity to DNA damaging agents
To introduce the CRISPR/Cas9 gene cassette, plants were transformed using Agrobacterium tumefaciens-mediated transformation. The CRISPR/Cas9 machinery introduced a single base pair insertion within the coding sequence of the ATR gene, which caused a frameshift mutation. Transformants containing the gene cassette and the desired mutations were selected, and by means of conventional crossing with wild-type plants, the CRISPR/Cas9 gene cassette was removed by selecting T1 plants that only contained the desired mutation, but no longer contained the gene cassette (null-segregants). Therefore, the plants in the field trial are not expected to contain foreign genetic material. They should only contain the desired mutation.
EN
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BCH-GENE-SCBD-115116-2 ATR serine/threonine kinase | Zea mays (Maize, Corn, MAIZE)Protein coding sequence | Impaired DNA repair,Sensitivity to DNA damaging agents
The gene has been edited using the CRISPR/Cas9 system resulting in a frameshift mutation. Therefore, the full length protein is not expected to be present within the modified plants.
EN
EN
Please note that two additional lines were also created to knock-out ATR activity. One contains the full deletion of the gene and the other a single basepair insertion in a different location to also cause a frameshift mutation.
Primarily, ATM responds to double stranded breaks, whereas ATR is activated by single stranded DNA and defects in replication fork progression.
EN
Primarily, ATM responds to double stranded breaks, whereas ATR is activated by single stranded DNA and defects in replication fork progression.
- Addgene: pBUN411 [ English ]
- GMOInfo (JRC|EU): Notification B/BE/19/V1 [ English ]
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