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Genetic element
(GENE)
published: 10 Dec 2020
last updated: 16 Dec 2020
R-2,4-dichlorophenoxypropionate dioxygenase
EN
-
RdpaEN
CS-ft_t-SPHHE
EN
Protein coding sequence
Yes
-
BCH-ORGA-SCBD-104811-3 Organism Sphingobium herbicidovorans (SPHHE)Bacteria
The protein sequence of (R)-dichlorprop dioxygenase was used as a scaffold in the engineering of the protein.
EN
EN
The Sphingobium herbicidovorans R-2,4-dichlorophenoxypropionate dioxygenase was engineered to have increased thermostability and enzymatic activity. The enzyme inactivates quizalofop (active against monocots) and 2,4-dichlorophenoxyacetic acid herbicides using α-ketoglutarate and molecular oxygen to produce the inactivated herbicide, succinate and carbon dioxide. The enzyme may also act on other aryloxyphenoxypropionate and synthetic auxin herbicides.
EN
EN
- Development of enzymes for robust aryloxyphenopropionate and synthetic auxin herbicide tolerance in maize and soybeans crops.pdf [ English ]
- GenBank - Synthetic construct FT_T_m gene, complete cds [ English ]
- Purification and characterization of two enantioselective 𝛼-ketoglutarate dependent dioxygenases, RdpA and SdpA, from Sphingomonas herbicidovorans.pdf [ English ]
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