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Living Modified Organism (LMO)
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FLAVR SAVR™ tomato
EN
Several lines
Yes
CGN-89564-2
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Organization:Monsanto (Calgene) ()Phone:Fax:Email:Website: http://www.monsato.com,
The FLAVR SAVR™ tomato lines were genetically engineered to express delayed softening by insertion of a PG encoding gene in the “antisense” orientation, which causes the endogenous PG messenger RNA (mRNA) to be degraded and, consequently, less production of PG protein.
The measured level of endogenous PG activity in transgenic FLAVR SAVR tomato was found to be less than 1% of PG activity found in the unmodified recipient organism.
EN
The measured level of endogenous PG activity in transgenic FLAVR SAVR tomato was found to be less than 1% of PG activity found in the unmodified recipient organism.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12079-5 Organism Solanum lycopersicum (Tomato, SOLLC)Crops
EN
Any of: pCGN1436, pCGN1547, pCGN1548, pCGN1549, pCGN1557, pCGN1558, pCGN1559, pCGN1578 or pCGN4109 depending on the line
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-15015-6 Polygalacturonase gene | Solanum lycopersicum (Tomato, SOLLC)Protein coding sequence | Changes in physiology and/or production (Ripening)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-45875-7 Beta-galactosidase gene | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101417-2 Mannopine Synthase Gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-101418-2 Mannopine synthase gene terminator | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-104340-2 Tumour Morphology Large gene terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)Terminator
Notes about the Other(s) sequence(s) specific to this LMO
The pg gene from tomato was introduced in the anti-sense orientation to suppress the expression of the endogenous pg gene.
Depending on the construct/vector used (see below), the antisense pg gene was under regulatory control of a single or two tandem copies of the 35S promoter from the cauliflower mosaic virus (CaMV). The terminator sequences for the antisense pg gene were the tml gene (T-tml) and the transcript 7 gene from the octopine-type Ti plasmid pTiA6 from A. tumefaciens.
Expression of the nptII gene was under the control of the 5' promoter and 3' terminator sequences from the mannopine synthase gene derived from A. tumefaciens or the 35s promoter and T-tml terminator, depending on the construct introduced in each line.
The DNA cassettes in two of the vectors used are shown below. Information on the cassettes in other vectors used to modify FLAVR SAVR™ was not found.
DNA cassette in pCGN1436:
LB (0.58) | P-mas (0.68) >> nptII (0.98) >> T-mas (0.77) | lac (0.29) | P-35S (0.6) >> P-35S (0.6) << pg (1.6) << Space (0.012) >> T-tml (1.2) | lac (0.17) | RB (0.28)
DNA cassette in pCGN4109:
LB | T-tml << nptII << P-35S | T-tml >> pg >> P-35S (0.6) << P-35S (0.6) | RB
Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.
Some Flavr Savr tomato lines transformed with vector pCGN1436 are:
501-1436-1001, 502-1436-2021, 7B-1436-92, 22B-1436-215, 28B-1436-419, 28B-1436-425, 28B-1436-498, 501-1436-1035, 105F-1436-2018, 105F-1436-2035, 105F-1436-2049 and N73-1436-111.
Some Flavr Savr tomato lines transformed with vector pCGN4109 are:
35F-4109a-3023, 84F-4109a-148, 88F-4109a-2797, 121F-4109a-333, 121F-4109a-1071, 121F-4109a-1120, 137F-4109a-71, 138F-4109a-164, 519A-4109a-4527, 519A-4109a-4621, 519A-4109a-4676, 531A-4109a-2105, 531A-4109a-2270, 532A-4109a-5097, 585A-4109a-3604, 585A-4109a-3530, 540A-4109a-1739, 532A-4109a-5166, 519A-4109a-4645, 540A-4109a-1823, 114F-4109a-26 and 114F-4109a-81.
EN
The pg gene from tomato was introduced in the anti-sense orientation to suppress the expression of the endogenous pg gene.
Depending on the construct/vector used (see below), the antisense pg gene was under regulatory control of a single or two tandem copies of the 35S promoter from the cauliflower mosaic virus (CaMV). The terminator sequences for the antisense pg gene were the tml gene (T-tml) and the transcript 7 gene from the octopine-type Ti plasmid pTiA6 from A. tumefaciens.
Expression of the nptII gene was under the control of the 5' promoter and 3' terminator sequences from the mannopine synthase gene derived from A. tumefaciens or the 35s promoter and T-tml terminator, depending on the construct introduced in each line.
The DNA cassettes in two of the vectors used are shown below. Information on the cassettes in other vectors used to modify FLAVR SAVR™ was not found.
DNA cassette in pCGN1436:
LB (0.58) | P-mas (0.68) >> nptII (0.98) >> T-mas (0.77) | lac (0.29) | P-35S (0.6) >> P-35S (0.6) << pg (1.6) << Space (0.012) >> T-tml (1.2) | lac (0.17) | RB (0.28)
DNA cassette in pCGN4109:
LB | T-tml << nptII << P-35S | T-tml >> pg >> P-35S (0.6) << P-35S (0.6) | RB
Note: the symbols >> and << indicate the sense and anti-sense direction of transcription, respectively, between different elements (typically, promoter - coding sequence - terminator). If present, the symbol | indicates elements that may be cloned adjacently in a cassette but that presumably are not transcribed together. The numbers in parenthesis indicate the approximate length in Kb of each genetic element.
Some Flavr Savr tomato lines transformed with vector pCGN1436 are:
501-1436-1001, 502-1436-2021, 7B-1436-92, 22B-1436-215, 28B-1436-419, 28B-1436-425, 28B-1436-498, 501-1436-1035, 105F-1436-2018, 105F-1436-2035, 105F-1436-2049 and N73-1436-111.
Some Flavr Savr tomato lines transformed with vector pCGN4109 are:
35F-4109a-3023, 84F-4109a-148, 88F-4109a-2797, 121F-4109a-333, 121F-4109a-1071, 121F-4109a-1120, 137F-4109a-71, 138F-4109a-164, 519A-4109a-4527, 519A-4109a-4621, 519A-4109a-4676, 531A-4109a-2105, 531A-4109a-2270, 532A-4109a-5097, 585A-4109a-3604, 585A-4109a-3530, 540A-4109a-1739, 532A-4109a-5166, 519A-4109a-4645, 540A-4109a-1823, 114F-4109a-26 and 114F-4109a-81.
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BCH-GENE-SCBD-15015-6 Polygalacturonase gene | Solanum lycopersicum (Tomato, SOLLC)Protein coding sequence | Changes in physiology and/or production (Ripening)
As a result of the introduction of a pg gene in the anti-sense orientation, the expression of the endogenous pg gene was suppressed.
The expression of other genes may have been affected in FLAVR SAVR™ tomato as a result of the transformation.
EN
The expression of other genes may have been affected in FLAVR SAVR™ tomato as a result of the transformation.
- Food
EN
EN
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