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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ Flex™ cotton
EN
MON88913
Yes
MON-88913-8
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
Cotton (Gossypium hirsutum) was modified to tolerate allow the use of glyphosate, the active ingredient in the herbicide Roundup®, and thus allows the herbicide's use a postemergent weed management option in cotton production. To achieve tolerance to glyphosate, Roundup Ready® Flex cotton (MON88913) expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which is a variant of a plant enzyme involved in the biosynthesis of aromatic amino acids and other metabolites in the shikimate pathway. The bacterial variant does not bind the herbicide with high affinity and thus allows for the continued functioning of the shikimate pathway upon exposure to overwise lethal applications of glyphosate. Two epsps cassettes were incorporated into the modified cotton to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)Crops
Variety: ‘Coker 312’
EN
PV-GHGT35
EN
- Agrobacterium-mediated DNA transfer
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0.000 kb
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1.039 kb
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0.045 kb
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0.621 kb
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0.227 kb
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1.367 kb
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0.642 kb
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0.000 kb
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1.174 kb
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0.140 kb
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0.472 kb
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0.227 kb
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1.367 kb
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0.642 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Leader
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BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-103907-3 Actin 8 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-103908-4 Actin 8 Leader sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Leader sequence
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BCH-GENE-SCBD-103909-3 Actin 8 Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
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BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Leader
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BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)Leader
Information on the inserted DNA sequences
The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two codon-optimised Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes:
(1) The first epsps coding sequence under the regulation of a chimeric transcriptional promoter (Figwort mosaic virus 34S promoter enhancer and Arabidopsis thaliana elongation factor EF-1 alpha (tsf1) promoter), tsf1 leader and intron sequences, an A. thaliana chloroplast transit peptide 2 sequence and a Pisum sativum ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) E9 transcript termination and polyadenylation sequence (T-E9).
(2) The second epsps coding sequence regulated by another chimeric transcriptional promoter (Cauliflower mosaic virus 35S enhancer and A. thaliana actin 8 (act8) promoter), act8 leader and intron sequences, A. thaliana chloroplast targeting peptide 2 and T-E9.
Note:
The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two codon-optimised Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes:
(1) The first epsps coding sequence under the regulation of a chimeric transcriptional promoter (Figwort mosaic virus 34S promoter enhancer and Arabidopsis thaliana elongation factor EF-1 alpha (tsf1) promoter), tsf1 leader and intron sequences, an A. thaliana chloroplast transit peptide 2 sequence and a Pisum sativum ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) E9 transcript termination and polyadenylation sequence (T-E9).
(2) The second epsps coding sequence regulated by another chimeric transcriptional promoter (Cauliflower mosaic virus 35S enhancer and A. thaliana actin 8 (act8) promoter), act8 leader and intron sequences, A. thaliana chloroplast targeting peptide 2 and T-E9.
Note:
- High levels of transcription are expected from both cassettes due to the presence of viral enhancer sequences. The EPSPS protein is expected to accumulate in the chloroplast due to the transit signal peptide.
- The promoter for both genetic constructs are chimeric promoters containing viral enhancer sequences. Thus, the size of the promoters (Elongation factor 1 alpha and Actin 8) in the 'Genetic elements construct' reflects the size of chimeric promoters (Figwort mosaic virus 34S enhancer + Elongation factor 1 alpha promoter; Cauliflower mosaic virus 35S enhancer + Actin 8 promoter).
- Southern blot analysis indicated the cotton contains a single T-DNA insertion and PV-GHGT35 backbone sequences were not inserted into the parental cotton genome.
EN
- Fiber/textile
- MON-88913-8 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-88913-8 - CropLife International Detection Methods Database [ English ]
- MON-88913-8 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-88913-8 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
The EPSPS enzyme is part of the shikimate pathway, an important biochemical pathway in plants involved in the production of aromatic amino acids and other aromatic compounds. The enzyme catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. When non-modified plants or weeds are treated with glyphosate, the plants cannot produce the aromatic amino acids needed for growth and survival. EPSPS is present in all plants, bacteria and fungi. Thus, EPSPS is naturally present in foods derived from plant and microbial sources. However, the protein is not present in animals, since these organisms are unable to synthesize their own aromatic amino acids.
- MON 88913-8 - APHIS [ English ]
- Euginius: MON88913 [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 48 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 46 | |
| Laboratory for detection and identification of LMOs | LMO(s) detectable by the laboratory | 7 | |
| Living Modified Organism | Recipient Organism or Parental Organisms | 11 | |
