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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect resistant, herbicide tolerant maize
EN
DBN9501
No
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Organization:Beijing Dabeinong Biotechnology Co., Ltd. (DBNBC)Private sector (business and industry)No. 2, Old Summer Palace West RoadBeijing, Haidan
100193, ChinaPhone: +86 010-82408600,Fax: +86 010-82408644,Email: hr-biotech@dbn.com.cn, cwbgs@dbn.com.cn,Website: http://www.dbnbc.cn/en,
The maize (Zea mays) was modified for insect resistance and herbicide tolerance. For resistance to Lepidoptera pests, the maize expresses Bacillus thuringiensis vegetative insecticidal protein 3Aa19 (a derivative of vegetative protein 3A), which causes gut paralysis and lysis of epithelium cells of larvae. For tolerance to glufinosate, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
EN
DBN10707
EN
- Agrobacterium-mediated DNA transfer
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0.168 kb
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0.253 kb
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0.552 kb
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1.993 kb
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0.253 kb
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2.370 kb
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0.806 kb
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0.639 kb
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0.293 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-15002-4 Phosphinothricin N-acetyltransferase gene | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-14990-5 Vegetative insecticidal protein 3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100359-7 Hsp70 intron | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified maize contains two gene cassettes: Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat) and Bacillus thuringiensis vegetative insecticidal protein 3Aa19 (vip3Aa19).
The pat coding sequence is under control of a maize ubiquitin 1 promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the constitutive nature of the promoter.
The vip3Aa19 sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and an A. tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the enhanced promoter and a maize heat shock protein 70 intron, which is also present to further enhance the expression of the coding sequence.
Note
- the vip3Aa19 sequence is a derivative of the vip3A sequence. The vip3Aa19 sequence has been codon optimized for expression in plants and contains an amino acid substitution (lysine was replaced with glutamate at position 284).
The pat coding sequence is under control of a maize ubiquitin 1 promoter and an Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the constitutive nature of the promoter.
The vip3Aa19 sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and an A. tumefaciens nopaline synthase terminator. High levels of transcription are expected due to the enhanced promoter and a maize heat shock protein 70 intron, which is also present to further enhance the expression of the coding sequence.
Note
- the vip3Aa19 sequence is a derivative of the vip3A sequence. The vip3Aa19 sequence has been codon optimized for expression in plants and contains an amino acid substitution (lysine was replaced with glutamate at position 284).
EN
- Feed
- Food
EN
EN
