Adeno-associated dependoparvovirus A (Adeno-associated virus 2) | BCH-ORGA-SCBD-260316 | Organism | Biosafety Clearing-House

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Organism (ORGA)

last updated: 28 Apr 2022
Organism information
Adeno-associated dependoparvovirus A
Kingdom Shotokuvirae
Phylum Cossaviricota
Class Quintoviricetes
Order Piccovirales
Family Parvoviridae
Subfamily Parvovirinae
Genus Dependoparvovirus
Species Adeno-associated dependoparvovirus A
  • AAV2
  • Adeno-associated virus 2
  • Adeno-associated virus 2
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Viruses
Characteristics related to biosafety
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Adeno-associate viruses (AAV) are defective, only undergoing productive replication in cells that are co-infected with another DNA virus, most commonly an adenovirus, which modifies the host environment in multiple ways that are essential to enable the AAV life cycle; by themselves AAVs are not associated with any known pathology. Under certain conditions, for example in the presence of DNA damaging agents, limited AAV replication can also be detected in the absence of helper viruses. Co-infection with helper virus triggers a lytic cycle as do some agents which appropriately perturb host cells. Wild-type AAV integrates preferentially into human chromosome 19q13.3-qter; recombinant vectors lose this specificity and appear to integrate randomly, thereby posing a theoretical risk of insertional mutagenesis.
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  • Pharmaceutical
  • Research
  • Other (Pathogen)
Additional Information
Adeno-associated virus 2 is a naked, icosahedral (20 - 25 nm diameter), single-stranded DNA virus. The genome consists of a single chromosome of 4679 nucleotides, flanked terminal repeats of 145 nucleotides that terminate in 125 nucleotide T-shaped hairpins (homotelomeric). These inverted repeats facilitate the integration into the host genome. As such, they have been employed in recombinant vectors to insert transgenes into the host genome. Thus, they may have a role in gene therapy.
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