Loading...
This record has been deleted. Since it is linked to a published record, it is available here for reference purposes only.
Click here to view the deleted recordThis document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Genetic element
(GENE)
last updated: 30 May 2022
Intron-encoded endonuclease
EN
CS-ppoI-PHYPO
EN
Protein coding sequence
No
-
BCH-ORGA-SCBD-260478-1 Organism Physarum polycephalum (Plasmodial slime molds, Slime mold, PHYPO)Slime mold
EN
Intron-encoded endonuclease I-PpoI
EN
See below
EN
PpoI is an intron-encoded endonuclease that specifically cleaves (*) double stranded DNA at the following recognition site:
5'- CTCTC TTAA*GGTAGC-3'
3'-GAGAG*AATT CCATCG-5'
The endonuclease creates staggered cuts with 5'-TTAA-3' overhangs. The enzyme itself functions as a homodimer and each submit has a molecular weight of 20 kDa (40 kDa as a homodimer).The target sequence can be found in ribosomal DNA repeats on the X chromosomes of certain insects and chromosome 12 of Saccharomyces cerevisiae. In biotechnology, this specific cutting on the X chromosome of insects could be utilized to promote a male sex bias (sex distorter drive) or male sterility.
5'- CTCTC TTAA*GGTAGC-3'
3'-GAGAG*AATT CCATCG-5'
The endonuclease creates staggered cuts with 5'-TTAA-3' overhangs. The enzyme itself functions as a homodimer and each submit has a molecular weight of 20 kDa (40 kDa as a homodimer).The target sequence can be found in ribosomal DNA repeats on the X chromosomes of certain insects and chromosome 12 of Saccharomyces cerevisiae. In biotechnology, this specific cutting on the X chromosome of insects could be utilized to promote a male sex bias (sex distorter drive) or male sterility.
- Interaction of the intron-encoded mobility endonuclease I-PpoI with its target site.pdf [ English ]
- UniProtKB - Intron-encoded endonuclease I-PpoI Q94702 (PPO1_PHYPO) [ English ]
- Digestion of I-PpoI Recognition Sites in Unfavorable Sequence Contexts Achieved by Changing the Reaction Conditions.pdf [ English ]
- A synthetic sex ratio distortion system for the control of the human malaria mosquito.pdf [ English ]
- DNA binding and cleavage by the nuclear intron-encoded endonuclease I-PpoI.pdf [ English ]
- GenBank - Physarum polycephalum intron 3 (I-Ppo) encoded endonuclease gene, complete cds [ English ]
Loading...
